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Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor.

Publication ,  Journal Article
Osada, T; Nagawa, H; Shibata, Y
Published in: J Immune Based Ther Vaccines
July 13, 2004

BACKGROUND: alpha-Galactosylceramide (alpha-GalCer) can be presented by CD1d molecules of antigen-presenting cells, and is known to induce a potent NKT cell-dependent cytotoxic response against tumor cells. However, the main effector cells in alpha-GalCer-induced antitumor immunity are still controversial. METHODS: In order to elucidate the cell phenotype that plays the most important role in alpha-GalCer-induced antitumor immunity, we purified and analyzed tumor-infiltrating leukocytes (TILs) from liver metastatic nodules of a colon cancer cell line (Colon26), comparing alpha-GalCer- and control vehicle-treated mice. Flow cytometry was performed to analyze cell phenotype in TILs and IFN-gamma ELISA was performed to detect antigen-specific immune response. RESULTS: Flow cytometry analysis showed a significantly higher infiltration of NK cells (DX5+, T cell receptor alphabeta (TCR)-) into tumors in alpha-GalCer-treated mice compared to vehicle-treated mice. The DX5+TCR+ cell population was not significantly different between these two groups, indicating that these cells were not the main effector cells. Interestingly, the CD8+ T cell population was increased in TILs of alpha-GalCer-treated mice, and the activation level of these cells based on CD69 expression was higher than that in vehicle-treated mice. Moreover, the number of tumor-infiltrating dendritic cells (DCs) was increased in alpha-GalCer-treated mice. IFN-gamma ELISA showed stronger antigen-specific response in TILs from alpha-GalCer-treated mice compared to those from vehicle-treated mice, although the difference between these two groups was not significant. CONCLUSIONS: In alpha-GalCer-induced antitumor immunity, NK cells seem to be some of the main effector cells and both CD8+ T cells and DCs, which are related to acquired immunity, might also play important roles in this antitumor immune response. These results suggest that alpha-GalCer has a multifunctional role in modulation of the immune response.

Duke Scholars

Published In

J Immune Based Ther Vaccines

DOI

ISSN

1476-8518

Publication Date

July 13, 2004

Volume

2

Issue

1

Start / End Page

7

Location

England

Related Subject Headings

  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
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Osada, T., Nagawa, H., & Shibata, Y. (2004). Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor. J Immune Based Ther Vaccines, 2(1), 7. https://doi.org/10.1186/1476-8518-2-7
Osada, Takuya, Hirokazu Nagawa, and Yoichi Shibata. “Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor.J Immune Based Ther Vaccines 2, no. 1 (July 13, 2004): 7. https://doi.org/10.1186/1476-8518-2-7.
Osada T, Nagawa H, Shibata Y. Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor. J Immune Based Ther Vaccines. 2004 Jul 13;2(1):7.
Osada, Takuya, et al. “Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor.J Immune Based Ther Vaccines, vol. 2, no. 1, July 2004, p. 7. Pubmed, doi:10.1186/1476-8518-2-7.
Osada T, Nagawa H, Shibata Y. Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor. J Immune Based Ther Vaccines. 2004 Jul 13;2(1):7.

Published In

J Immune Based Ther Vaccines

DOI

ISSN

1476-8518

Publication Date

July 13, 2004

Volume

2

Issue

1

Start / End Page

7

Location

England

Related Subject Headings

  • Immunology