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Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats.

Publication ,  Journal Article
Nakade, Y; Tsukamoto, K; Iwa, M; Pappas, TN; Takahashi, T
Published in: Auton Neurosci
January 30, 2007

Glucagon like peptide-1 (7-36) (GLP-1), one of the gastrointestinal (GI) regulatory peptide, is known to act as a stress related brain neurotransmitter mediating GI function. Central administration of GLP-1 inhibits gastric emptying. However, little is known about the effect of central GLP-1 on colonic transit. Effects and mechanism of GLP-1 on colonic transit were investigated in conscious rats. Immediately after intracerebroventricular (icv)-injection of GLP-1, 51Cr was applied via the catheter positioned to the proximal colon. 90 min after 51Cr injection, rats were euthanized and the colon was removed and divided into 10 equal segments. The radioactivity of each segment was counted and the geometric center (GC) was calculated. Icv-injection of GLP-1 (0.3-3 nmol) dose-dependently accelerated colonic transit [(GC: 4.4+/-0.2 in controls, 7.8+/-0.5 in GLP-1 (3 nmol)]. In contrast, intraperitoneal (ip)-injection of GLP-1 (3 nmol) did not modify colonic transit. Icv-injection of GLP-1 (3 nmol)-induced acceleration of colonic transit was attenuated by vagotomy, atropine and hexamethonium, but not by guanethidine. Icv-injection of GLP-1 (3 nmol)-induced acceleration of colonic transit was abolished by corticotropin releasing factor (CRF) antagonist, astressin. Restraint stress-induced acceleration of colonic transit was abolished by a selective GLP-1 receptor antagonist, exendin. These results indicate that the endogenous GLP-1 is involved in mediating stress-induced alteration of colonic transit via a central CRF and peripheral cholinergic pathways in rats.

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Published In

Auton Neurosci

DOI

ISSN

1566-0702

Publication Date

January 30, 2007

Volume

131

Issue

1-2

Start / End Page

50 / 56

Location

Netherlands

Related Subject Headings

  • Vagus Nerve
  • Vagotomy
  • Rats, Sprague-Dawley
  • Rats
  • Peptide Fragments
  • Neurology & Neurosurgery
  • Neural Pathways
  • Male
  • Glucagon-Like Peptide 1
  • Gastrointestinal Motility
 

Citation

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Nakade, Y., Tsukamoto, K., Iwa, M., Pappas, T. N., & Takahashi, T. (2007). Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats. Auton Neurosci, 131(1–2), 50–56. https://doi.org/10.1016/j.autneu.2006.06.007
Nakade, Yukiomi, Kiyoshi Tsukamoto, Masahiro Iwa, Theodore N. Pappas, and Toku Takahashi. “Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats.Auton Neurosci 131, no. 1–2 (January 30, 2007): 50–56. https://doi.org/10.1016/j.autneu.2006.06.007.
Nakade Y, Tsukamoto K, Iwa M, Pappas TN, Takahashi T. Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats. Auton Neurosci. 2007 Jan 30;131(1–2):50–6.
Nakade, Yukiomi, et al. “Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats.Auton Neurosci, vol. 131, no. 1–2, Jan. 2007, pp. 50–56. Pubmed, doi:10.1016/j.autneu.2006.06.007.
Nakade Y, Tsukamoto K, Iwa M, Pappas TN, Takahashi T. Glucagon like peptide-1 accelerates colonic transit via central CRF and peripheral vagal pathways in conscious rats. Auton Neurosci. 2007 Jan 30;131(1–2):50–56.
Journal cover image

Published In

Auton Neurosci

DOI

ISSN

1566-0702

Publication Date

January 30, 2007

Volume

131

Issue

1-2

Start / End Page

50 / 56

Location

Netherlands

Related Subject Headings

  • Vagus Nerve
  • Vagotomy
  • Rats, Sprague-Dawley
  • Rats
  • Peptide Fragments
  • Neurology & Neurosurgery
  • Neural Pathways
  • Male
  • Glucagon-Like Peptide 1
  • Gastrointestinal Motility