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Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection.

Publication ,  Journal Article
White, IR; Patel, K; Symonds, WT; Dev, A; Griffin, P; Tsokanas, N; Skehel, M; Liu, C; Zekry, A; Cutler, P; Gattu, M; Rockey, DC; Berrey, MM ...
Published in: J Transl Med
July 11, 2007

BACKGROUND: Despite its widespread use to assess fibrosis, liver biopsy has several important drawbacks, including that is it semi-quantitative, invasive, and limited by sampling and observer variability. Non-invasive serum biomarkers may more accurately reflect the fibrogenetic process. To identify potential biomarkers of fibrosis, we compared serum protein expression profiles in patients with chronic hepatitis C (CHC) virus infection and fibrosis. METHODS: Twenty-one patients with no or mild fibrosis (METAVIR stage F0, F1) and 23 with advanced fibrosis (F3, F4) were retrospectively identified from a pedigreed database of 1600 CHC patients. All samples were carefully phenotyped and matched for age, gender, race, body mass index, genotype, duration of infection, alcohol use, and viral load. Expression profiling was performed in a blinded fashion using a 2D polyacrylamide gel electrophoresis/LC-MS/MS platform. Partial least squares discriminant analysis and likelihood ratio statistics were used to rank individual differences in protein expression between the 2 groups. RESULTS: Seven individual protein spots were identified as either significantly increased (alpha2-macroglobulin, haptoglobin, albumin) or decreased (complement C-4, serum retinol binding protein, apolipoprotein A-1, and two isoforms of apolipoprotein A-IV) with advanced fibrosis. Three individual proteins, haptoglobin, apolipoprotein A-1, and alpha2-macroglobulin, are included in existing non-invasive serum marker panels. CONCLUSION: Biomarkers identified through expression profiling may facilitate the development of more accurate marker algorithms to better quantitate hepatic fibrosis and monitor disease progression.

Duke Scholars

Published In

J Transl Med

DOI

EISSN

1479-5876

Publication Date

July 11, 2007

Volume

5

Start / End Page

33

Location

England

Related Subject Headings

  • Proteomics
  • Principal Component Analysis
  • Male
  • Liver Cirrhosis
  • Immunology
  • Humans
  • Hepatitis C
  • Female
  • Electrophoresis, Gel, Two-Dimensional
  • Disease Progression
 

Citation

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White, I. R., Patel, K., Symonds, W. T., Dev, A., Griffin, P., Tsokanas, N., … McHutchison, J. G. (2007). Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection. J Transl Med, 5, 33. https://doi.org/10.1186/1479-5876-5-33
White, Ian R., Keyur Patel, William T. Symonds, Anouk Dev, Philip Griffin, Nikos Tsokanas, Mark Skehel, et al. “Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection.J Transl Med 5 (July 11, 2007): 33. https://doi.org/10.1186/1479-5876-5-33.
White IR, Patel K, Symonds WT, Dev A, Griffin P, Tsokanas N, et al. Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection. J Transl Med. 2007 Jul 11;5:33.
White, Ian R., et al. “Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection.J Transl Med, vol. 5, July 2007, p. 33. Pubmed, doi:10.1186/1479-5876-5-33.
White IR, Patel K, Symonds WT, Dev A, Griffin P, Tsokanas N, Skehel M, Liu C, Zekry A, Cutler P, Gattu M, Rockey DC, Berrey MM, McHutchison JG. Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection. J Transl Med. 2007 Jul 11;5:33.
Journal cover image

Published In

J Transl Med

DOI

EISSN

1479-5876

Publication Date

July 11, 2007

Volume

5

Start / End Page

33

Location

England

Related Subject Headings

  • Proteomics
  • Principal Component Analysis
  • Male
  • Liver Cirrhosis
  • Immunology
  • Humans
  • Hepatitis C
  • Female
  • Electrophoresis, Gel, Two-Dimensional
  • Disease Progression