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Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis.

Publication ,  Journal Article
Perfect, JR; Magee, BB; Magee, PT
Published in: Infect Immun
September 1989

Chromosomes from Cryptococcus neoformans, an encapsulated yeast pathogen, were separated by contour-clamped homogeneous field gel electrophoresis. Seven strains representing all four serotypes were studied. It was found that each strain had a unique, reproducible pattern of chromosome bands which could potentially be used for strain polymorphism studies. There were between 10 and 12 chromosomes in the strains studied, with an approximate genomic size of 15,000 to 17,000 kilobases. Chromosome separation also could be used to assign locations for cloned genes, and the ribosomal DNA genes were found on one of the larger C. neoformans chromosomes. The technique of electrophoretic karyotyping should be helpful for genetic and molecular investigations into the biology of C. neoformans.

Duke Scholars

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 1989

Volume

57

Issue

9

Start / End Page

2624 / 2627

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Microbiology
  • Karyotyping
  • Genes, Fungal
  • Electrophoresis, Agar Gel
  • DNA, Fungal
  • Cryptococcus neoformans
  • Cryptococcus
  • Chromosomes
  • Chromosome Mapping
 

Citation

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Perfect, J. R., Magee, B. B., & Magee, P. T. (1989). Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun, 57(9), 2624–2627. https://doi.org/10.1128/iai.57.9.2624-2627.1989
Perfect, J. R., B. B. Magee, and P. T. Magee. “Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis.Infect Immun 57, no. 9 (September 1989): 2624–27. https://doi.org/10.1128/iai.57.9.2624-2627.1989.
Perfect JR, Magee BB, Magee PT. Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun. 1989 Sep;57(9):2624–7.
Perfect, J. R., et al. “Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis.Infect Immun, vol. 57, no. 9, Sept. 1989, pp. 2624–27. Pubmed, doi:10.1128/iai.57.9.2624-2627.1989.
Perfect JR, Magee BB, Magee PT. Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun. 1989 Sep;57(9):2624–2627.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 1989

Volume

57

Issue

9

Start / End Page

2624 / 2627

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Microbiology
  • Karyotyping
  • Genes, Fungal
  • Electrophoresis, Agar Gel
  • DNA, Fungal
  • Cryptococcus neoformans
  • Cryptococcus
  • Chromosomes
  • Chromosome Mapping