High rates of "unselected" aneuploidy and chromosome rearrangements in tel1 mec1 haploid yeast strains.
The yeast TEL1 and MEC1 genes (homologous to the mammalian ATM and ATR genes, respectively) serve partially redundant roles in the detection of DNA damage and in the regulation of telomere length. Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell divisions. The subcultured strains had very high rates of chromosome aberrations: duplications, deletions, and translocations. The breakpoints of the rearranged chromosomes were within retrotransposons (Ty or delta-repeats), and these chromosome aberrations nonrandomly involved chromosome III. In addition, we showed that strains with the hypomorphic mec1-21 allele often became disomic for chromosome VIII. This property of the mec1-21 strains is suppressed by a plasmid containing the DNA2 gene (located on chromosome VIII) that encodes an essential nuclease/helicase involved in DNA replication and DNA repair.
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Related Subject Headings
- Species Specificity
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Protein Serine-Threonine Kinases
- Microarray Analysis
- Intracellular Signaling Peptides and Proteins
- Developmental Biology
- DNA Repair
- DNA Primers
- Chromosome Aberrations
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Species Specificity
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Protein Serine-Threonine Kinases
- Microarray Analysis
- Intracellular Signaling Peptides and Proteins
- Developmental Biology
- DNA Repair
- DNA Primers
- Chromosome Aberrations