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The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks.

Publication ,  Journal Article
Narayanan, V; Mieczkowski, PA; Kim, H-M; Petes, TD; Lobachev, KS
Published in: Cell
June 30, 2006

DNA palindromes often colocalize in cancer cells with chromosomal regions that are predisposed to gene amplification. The molecular mechanisms by which palindromes can cause gene amplification are largely unknown. Using yeast as a model system, we found that hairpin-capped double-strand breaks (DSBs) occurring at the location of human Alu-quasipalindromes lead to the formation of intrachromosomal amplicons with large inverted repeats (equivalent to homogeneously staining regions in mammalian chromosomes) or extrachromosomal palindromic molecules (equivalent to double minutes [DM] in mammalian cells). We demonstrate that the specific outcomes of gene amplification depend on the applied selection, the nature of the break, and the chromosomal location of the amplified gene relative to the site of the hairpin-capped DSB. The rules for the palindrome-dependent pathway of gene amplification defined in yeast may operate during the formation of amplicons in human tumors.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

June 30, 2006

Volume

125

Issue

7

Start / End Page

1283 / 1296

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Nucleic Acid Conformation
  • Neoplasms
  • Models, Genetic
  • Humans
  • Gene Rearrangement
  • Gene Amplification
  • Developmental Biology
  • DNA, Neoplasm
 

Citation

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Narayanan, V., Mieczkowski, P. A., Kim, H.-M., Petes, T. D., & Lobachev, K. S. (2006). The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks. Cell, 125(7), 1283–1296. https://doi.org/10.1016/j.cell.2006.04.042
Narayanan, Vidhya, Piotr A. Mieczkowski, Hyun-Min Kim, Thomas D. Petes, and Kirill S. Lobachev. “The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks.Cell 125, no. 7 (June 30, 2006): 1283–96. https://doi.org/10.1016/j.cell.2006.04.042.
Narayanan V, Mieczkowski PA, Kim H-M, Petes TD, Lobachev KS. The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks. Cell. 2006 Jun 30;125(7):1283–96.
Narayanan, Vidhya, et al. “The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks.Cell, vol. 125, no. 7, June 2006, pp. 1283–96. Pubmed, doi:10.1016/j.cell.2006.04.042.
Narayanan V, Mieczkowski PA, Kim H-M, Petes TD, Lobachev KS. The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks. Cell. 2006 Jun 30;125(7):1283–1296.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

June 30, 2006

Volume

125

Issue

7

Start / End Page

1283 / 1296

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Nucleic Acid Conformation
  • Neoplasms
  • Models, Genetic
  • Humans
  • Gene Rearrangement
  • Gene Amplification
  • Developmental Biology
  • DNA, Neoplasm