Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.
Publication
, Journal Article
Nag, DK; Petes, TD
Published in: Mol Cell Biol
April 1993
We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.
Duke Scholars
Published In
Mol Cell Biol
DOI
ISSN
0270-7306
Publication Date
April 1993
Volume
13
Issue
4
Start / End Page
2324 / 2331
Location
United States
Related Subject Headings
- Time Factors
- Spores, Fungal
- Saccharomyces cerevisiae
- Restriction Mapping
- Recombination, Genetic
- Oligodeoxyribonucleotides
- Nucleic Acid Hybridization
- Nucleic Acid Conformation
- Meiosis
- Genes, Fungal
Citation
APA
Chicago
ICMJE
MLA
NLM
Nag, D. K., & Petes, T. D. (1993). Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae. Mol Cell Biol, 13(4), 2324–2331. https://doi.org/10.1128/mcb.13.4.2324-2331.1993
Nag, D. K., and T. D. Petes. “Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.” Mol Cell Biol 13, no. 4 (April 1993): 2324–31. https://doi.org/10.1128/mcb.13.4.2324-2331.1993.
Nag DK, Petes TD. Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1993 Apr;13(4):2324–31.
Nag, D. K., and T. D. Petes. “Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.” Mol Cell Biol, vol. 13, no. 4, Apr. 1993, pp. 2324–31. Pubmed, doi:10.1128/mcb.13.4.2324-2331.1993.
Nag DK, Petes TD. Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1993 Apr;13(4):2324–2331.
Published In
Mol Cell Biol
DOI
ISSN
0270-7306
Publication Date
April 1993
Volume
13
Issue
4
Start / End Page
2324 / 2331
Location
United States
Related Subject Headings
- Time Factors
- Spores, Fungal
- Saccharomyces cerevisiae
- Restriction Mapping
- Recombination, Genetic
- Oligodeoxyribonucleotides
- Nucleic Acid Hybridization
- Nucleic Acid Conformation
- Meiosis
- Genes, Fungal