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Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.

Publication ,  Journal Article
Nag, DK; Petes, TD
Published in: Mol Cell Biol
April 1993

We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1993

Volume

13

Issue

4

Start / End Page

2324 / 2331

Location

United States

Related Subject Headings

  • Time Factors
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombination, Genetic
  • Oligodeoxyribonucleotides
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • Meiosis
  • Genes, Fungal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nag, D. K., & Petes, T. D. (1993). Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae. Mol Cell Biol, 13(4), 2324–2331. https://doi.org/10.1128/mcb.13.4.2324-2331.1993
Nag, D. K., and T. D. Petes. “Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.Mol Cell Biol 13, no. 4 (April 1993): 2324–31. https://doi.org/10.1128/mcb.13.4.2324-2331.1993.
Nag, D. K., and T. D. Petes. “Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.Mol Cell Biol, vol. 13, no. 4, Apr. 1993, pp. 2324–31. Pubmed, doi:10.1128/mcb.13.4.2324-2331.1993.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1993

Volume

13

Issue

4

Start / End Page

2324 / 2331

Location

United States

Related Subject Headings

  • Time Factors
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombination, Genetic
  • Oligodeoxyribonucleotides
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • Meiosis
  • Genes, Fungal