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Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos.

Publication ,  Journal Article
Wang, X; Wu, Y; Stonehuerner, JG; Dailey, LA; Richards, JD; Jaspers, I; Piantadosi, CA; Ghio, AJ
Published in: Am J Respir Cell Mol Biol
March 2006

Lung injury after asbestos exposure is associated with an oxidative stress that is catalyzed by iron in the fiber matrix, complexed to the surface, or both. We tested the hypothesis that the cellular response to asbestos includes the transport and sequestration of this iron through (1) generation of superoxide for ferrireduction, (2) up-regulation of divalent metal transporter-1 (DMT1) for intracellular transport of Fe2+, and (3) increased production of cellular ferritin where the metal is stored in a catalytically less reactive state. BEAS-2B cells with normal and elevated Cu,Zn superoxide dismutase (SOD) expression were employed for in vitro investigations. After exposure of these cells to asbestos, we demonstrated by fluorescence methodology a significantly increased generation of SOD with ferrireductive capacity. Fiber exposure also increased DMT1 protein and mRNA expression in the BEAS-2B cells. Incubation with asbestos elevated cellular iron and ferritin concentrations, and these responses were diminished in cells with an enhanced expression of SOD. Finally, fiber exposure increased supernatant concentrations of interleukin 8, but this inflammatory mediator was actually increased in cells with elevated SOD expression. We conclude that the response of respiratory epithelial cells to asbestos includes oxidant-mediated mechanisms to sequester catalytically active iron associated with the fiber.

Duke Scholars

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

March 2006

Volume

34

Issue

3

Start / End Page

286 / 292

Location

United States

Related Subject Headings

  • Superoxides
  • Superoxide Dismutase
  • Respiratory System
  • Respiratory Mucosa
  • Oxidative Stress
  • Oxidation-Reduction
  • Oxidants
  • Mineral Fibers
  • Iron
  • Interleukin-8
 

Citation

APA
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Wang, X., Wu, Y., Stonehuerner, J. G., Dailey, L. A., Richards, J. D., Jaspers, I., … Ghio, A. J. (2006). Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos. Am J Respir Cell Mol Biol, 34(3), 286–292. https://doi.org/10.1165/rcmb.2004-0275OC
Wang, Xinchao, Yiming Wu, Jacqueline G. Stonehuerner, Lisa A. Dailey, Judy D. Richards, Ilona Jaspers, Claude A. Piantadosi, and Andrew J. Ghio. “Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos.Am J Respir Cell Mol Biol 34, no. 3 (March 2006): 286–92. https://doi.org/10.1165/rcmb.2004-0275OC.
Wang X, Wu Y, Stonehuerner JG, Dailey LA, Richards JD, Jaspers I, et al. Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos. Am J Respir Cell Mol Biol. 2006 Mar;34(3):286–92.
Wang, Xinchao, et al. “Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos.Am J Respir Cell Mol Biol, vol. 34, no. 3, Mar. 2006, pp. 286–92. Pubmed, doi:10.1165/rcmb.2004-0275OC.
Wang X, Wu Y, Stonehuerner JG, Dailey LA, Richards JD, Jaspers I, Piantadosi CA, Ghio AJ. Oxidant generation promotes iron sequestration in BEAS-2B cells exposed to asbestos. Am J Respir Cell Mol Biol. 2006 Mar;34(3):286–292.

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

March 2006

Volume

34

Issue

3

Start / End Page

286 / 292

Location

United States

Related Subject Headings

  • Superoxides
  • Superoxide Dismutase
  • Respiratory System
  • Respiratory Mucosa
  • Oxidative Stress
  • Oxidation-Reduction
  • Oxidants
  • Mineral Fibers
  • Iron
  • Interleukin-8