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Release of cytochrome c from liver mitochondria during permeability transition.

Publication ,  Journal Article
Kantrow, SP; Piantadosi, CA
Published in: Biochem Biophys Res Commun
March 27, 1997

The mitochondrial permeability transition (PT) follows opening of megachannels in the inner membrane and may be part of a programmed death pathway. Recently a role for cytochrome c in programmed cell death has been proposed, although its relationship to PT has not been been determined. We studied the release of cytochrome c from liver mitochondria undergoing PT. Well-coupled mitochondria treated with 5 mM atractyloside (ATR) or 100 microM calcium chloride were found to undergo PT and release cytochrome c into the incubation buffer within 5 minutes. Control mitochondria and mitochondria treated with the uncoupler FCCP did not undergo PT or release cytochrome c at 5 or 15 minutes. PT induced by ATR could be prevented by pretreatment with 10 microM cyclosporin A. Mitochondria incubated with ATR or calcium caused a 20-30% decrease in electron transfer rate via cytochrome c and cytochrome c oxidase. We conclude that cytochrome c release is an early event during mitochondrial PT, and is sufficient to decrease electron transfer through the terminal electron transport components of the mitochondrion.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 27, 1997

Volume

232

Issue

3

Start / End Page

669 / 671

Location

United States

Related Subject Headings

  • Uncoupling Agents
  • Rats, Sprague-Dawley
  • Rats
  • Permeability
  • Mitochondria, Liver
  • In Vitro Techniques
  • Electron Transport Complex IV
  • Electron Transport
  • Cytochrome c Group
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
 

Citation

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Kantrow, S. P., & Piantadosi, C. A. (1997). Release of cytochrome c from liver mitochondria during permeability transition. Biochem Biophys Res Commun, 232(3), 669–671. https://doi.org/10.1006/bbrc.1997.6353
Kantrow, S. P., and C. A. Piantadosi. “Release of cytochrome c from liver mitochondria during permeability transition.Biochem Biophys Res Commun 232, no. 3 (March 27, 1997): 669–71. https://doi.org/10.1006/bbrc.1997.6353.
Kantrow SP, Piantadosi CA. Release of cytochrome c from liver mitochondria during permeability transition. Biochem Biophys Res Commun. 1997 Mar 27;232(3):669–71.
Kantrow, S. P., and C. A. Piantadosi. “Release of cytochrome c from liver mitochondria during permeability transition.Biochem Biophys Res Commun, vol. 232, no. 3, Mar. 1997, pp. 669–71. Pubmed, doi:10.1006/bbrc.1997.6353.
Kantrow SP, Piantadosi CA. Release of cytochrome c from liver mitochondria during permeability transition. Biochem Biophys Res Commun. 1997 Mar 27;232(3):669–671.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 27, 1997

Volume

232

Issue

3

Start / End Page

669 / 671

Location

United States

Related Subject Headings

  • Uncoupling Agents
  • Rats, Sprague-Dawley
  • Rats
  • Permeability
  • Mitochondria, Liver
  • In Vitro Techniques
  • Electron Transport Complex IV
  • Electron Transport
  • Cytochrome c Group
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone