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Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells.

Publication ,  Journal Article
Crochet, JR; Shah, AA; Schomberg, DW; Price, TM
Published in: J Clin Endocrinol Metab
September 2012

CONTEXT: Trophoblast-derived human chorionic gonadotropin (hCG) promotes corpus luteum progesterone (P4) production, and wide ranges of serum P4 levels are noted in various pregnancy outcomes, despite similar hCG concentrations. There are five unique biologically active hCG variants in human pregnancy urine, and previous studies of P4 production in response to hCG have used only preparations containing all isoforms. Understanding exactly which hCG variant is primarily responsible for stimulating corpus luteum steroidogenesis may have great clinical and diagnostic implications, including in the setting of ectopic pregnancy. OBJECTIVE: Our objective was to delineate the role of the standard and hyperglycosylated (H)-hCG isoforms in stimulating P4 production by luteinized granulosa cells. DESIGN AND SETTING: Cell culture, ELISA, and fluorometric-based protein assays were done at Duke University Medical Center. PATIENTS: Patients were anonymous oocyte donors. INTERVENTION: Cultured luteinized granulosa cells were treated with 0.25, 0.5, and 1.0 ng/ml total hCG, which contains all isoforms, purified standard hCG (37.1 kDa), and purified H-hCG (42.8 kDa). MAIN OUTCOME MEASURE: P4 produced per total cellular protein (nanograms per microgram) was measured via ELISA and fluorometric protein determination kits. RESULTS: Both total hCG (P = 0.0003) and purified standard hCG (P < 0.0001) stimulated a dose-dependent increase in P4 production. Purified H-hCG did not change the P4 produced per total cellular protein response (P value not significant). CONCLUSIONS: Standard hCG stimulated P4 production by cultured granulosa cells and likely supports corpus luteum function via interactions with the LH/hCG receptor. In contrast, H-hCG did not increase P4 production, which indicates a nonsteroidogenic role for this protein during early gestation.

Duke Scholars

Published In

J Clin Endocrinol Metab

DOI

EISSN

1945-7197

Publication Date

September 2012

Volume

97

Issue

9

Start / End Page

E1741 / E1744

Location

United States

Related Subject Headings

  • Progesterone
  • Oocytes
  • Luteinization
  • Luteal Cells
  • Isomerism
  • Humans
  • Glycosylation
  • Fluorometry
  • Female
  • Enzyme-Linked Immunosorbent Assay
 

Citation

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ICMJE
MLA
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Crochet, J. R., Shah, A. A., Schomberg, D. W., & Price, T. M. (2012). Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells. J Clin Endocrinol Metab, 97(9), E1741–E1744. https://doi.org/10.1210/jc.2012-2027
Crochet, John R., Anish A. Shah, David W. Schomberg, and Thomas M. Price. “Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells.J Clin Endocrinol Metab 97, no. 9 (September 2012): E1741–44. https://doi.org/10.1210/jc.2012-2027.
Crochet JR, Shah AA, Schomberg DW, Price TM. Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells. J Clin Endocrinol Metab. 2012 Sep;97(9):E1741–4.
Crochet, John R., et al. “Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells.J Clin Endocrinol Metab, vol. 97, no. 9, Sept. 2012, pp. E1741–44. Pubmed, doi:10.1210/jc.2012-2027.
Crochet JR, Shah AA, Schomberg DW, Price TM. Hyperglycosylated human chorionic gonadotropin does not increase progesterone production by luteinized granulosa cells. J Clin Endocrinol Metab. 2012 Sep;97(9):E1741–E1744.
Journal cover image

Published In

J Clin Endocrinol Metab

DOI

EISSN

1945-7197

Publication Date

September 2012

Volume

97

Issue

9

Start / End Page

E1741 / E1744

Location

United States

Related Subject Headings

  • Progesterone
  • Oocytes
  • Luteinization
  • Luteal Cells
  • Isomerism
  • Humans
  • Glycosylation
  • Fluorometry
  • Female
  • Enzyme-Linked Immunosorbent Assay