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Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis

Publication ,  Journal Article
Vietorisz, EC; Rose, JG; Fowler, WC; Straub, KD; Barnett, GA; Proia, AD; Ross, JR; Iacobucci, MJ; Szarmes, EB; Plunkett, MA; Howell, DM
Published in: Investigative Ophthalmology and Visual Science
February 15, 1996

Purpose: To evaluate histologically the ablative properties of the mid-infrared range of wavelengths on corneal tissue, with specific regard to dimensions of ablation and extent of collateral damage. Methods: Free electron laser (FEL) generated wavelengths of 2-9 to 7.0μm at powers of 5, 10 and 15mJ were systematically delivered to the corneas of freshly-enucleated porcine globes. Fluence = 0.75, 1.51 and 2.26J/cm2, respectively. Spot size = 60 × 70μm. Macropulse duration was 4.0μs with frequency of 10Hz; micropulse duration was 1.0ps with frequency of 2.8GHz. Treated corneas were embedded in paraffin for H&E-stained sections. Results: H&E-stained paraffin sections through ablation zones reveal small amounts (5-10μm) of damage collateral to ablations at 2.94 and 3.4μm wavelengths, while no collateral damage is apparent at 6.0μm. Ablations performed using other wavelengths in the 2.9 to 7.0μm range possess 20-100μm of collateral damage. Conclusions: 1. The mid-IR FEL-generated wavelength demonstrating the least collateral distortion coincided with the resonance frequency for the amide bond, 6.0/6.45μm. 2. Minimal collateral effects were noted at resonance frequencies for the OH bond, 2.94μm, and CH bond, 3.4μm. 3. Ablation at selected IR wavelengths corresponding to biochemical bond resonance frequencies may have keratorefractive applcations.

Duke Scholars

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

February 15, 1996

Volume

37

Issue

3

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Vietorisz, E. C., Rose, J. G., Fowler, W. C., Straub, K. D., Barnett, G. A., Proia, A. D., … Howell, D. M. (1996). Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis. Investigative Ophthalmology and Visual Science, 37(3).
Vietorisz, E. C., J. G. Rose, W. C. Fowler, K. D. Straub, G. A. Barnett, A. D. Proia, J. R. Ross, et al. “Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis.” Investigative Ophthalmology and Visual Science 37, no. 3 (February 15, 1996).
Vietorisz EC, Rose JG, Fowler WC, Straub KD, Barnett GA, Proia AD, et al. Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Vietorisz, E. C., et al. “Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis.” Investigative Ophthalmology and Visual Science, vol. 37, no. 3, Feb. 1996.
Vietorisz EC, Rose JG, Fowler WC, Straub KD, Barnett GA, Proia AD, Ross JR, Iacobucci MJ, Szarmes EB, Plunkett MA, Howell DM. Systematic free electron laser delivery of mid-infrared wavelengths to porcine corneas: Histologic analysis. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

February 15, 1996

Volume

37

Issue

3

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences