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Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration.

Publication ,  Journal Article
Rao, PV; Zigler, JS
Published in: Biochem Biophys Res Commun
March 30, 1990

Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowledge the values reported are higher than have been observed in any tissue. A clear correlation exists between NADPH and zeta-crystallin contents of the lens both in normal guinea pigs during development and in a line of guinea pigs with a mutation in the gene for zeta-crystallin. Heterozygotes for this mutation had a 50% reduction in NADPH, while homozygotes have only about 6% of the normal level. NADP+ levels were also markedly elevated suggesting that redox cycling of the NADPH is occurring.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 30, 1990

Volume

167

Issue

3

Start / End Page

1221 / 1228

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Rats, Inbred Strains
  • Rats
  • Protein Binding
  • Phosphogluconate Dehydrogenase
  • Oxidation-Reduction
  • NADP
  • NAD
  • Lens, Crystalline
  • Guinea Pigs
 

Citation

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Rao, P. V., & Zigler, J. S. (1990). Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration. Biochem Biophys Res Commun, 167(3), 1221–1228. https://doi.org/10.1016/0006-291x(90)90654-6
Rao, P. V., and J. S. Zigler. “Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration.Biochem Biophys Res Commun 167, no. 3 (March 30, 1990): 1221–28. https://doi.org/10.1016/0006-291x(90)90654-6.
Rao PV, Zigler JS. Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration. Biochem Biophys Res Commun. 1990 Mar 30;167(3):1221–8.
Rao, P. V., and J. S. Zigler. “Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration.Biochem Biophys Res Commun, vol. 167, no. 3, Mar. 1990, pp. 1221–28. Pubmed, doi:10.1016/0006-291x(90)90654-6.
Rao PV, Zigler JS. Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration. Biochem Biophys Res Commun. 1990 Mar 30;167(3):1221–1228.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 30, 1990

Volume

167

Issue

3

Start / End Page

1221 / 1228

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Rats, Inbred Strains
  • Rats
  • Protein Binding
  • Phosphogluconate Dehydrogenase
  • Oxidation-Reduction
  • NADP
  • NAD
  • Lens, Crystalline
  • Guinea Pigs