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Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH.

Publication ,  Journal Article
Harris, JM; Lopez, GP; Reichert, WM
Published in: Sensors and actuators. B, Chemical
November 2012

The objectives of this study were to examine the feasibility of using glucose oxidase (GOx) dispersed in a silica matrix for glucose monitoring in whole blood, and then to assess whether the flexibility of silica sol-gel chemistry could be exploited to enhance glucose sensor performance and stability. Silica-dispersed GOx was deployed on platinized platinum (Pt) wire to form a Clark-type amperometric glucose sensor. Sensors were calibrated using buffered glucose standard solutions, and then tested against glucose spiked human serum and whole blood. All serum and whole blood measurements met the minimum FDA requirement of falling within the "A+B region" of a Clark Error Grid. To our knowledge this is the first report of using silica-dispersed GOx to measure glucose in whole blood. The effect of condensation pH on sensor performance was assessed by dispersing GOx in silica condensed at pH 3, 7 and 12, and then testing the sensor response against glucose calibration standards. The pH 12 silica sensors had statistically faster response time, and higher sensor sensitivity compared to pH 7, pH 3 silica and glutaraldehyde crosslinked sensors. Membranes of the pH 12 silica had statistically higher glucose diffusion coefficient than did the pH 7 and 3 sensors. GOx dispersed in pH 12 silica also had the longest half life. We hypothesize that the gel-like pH 12 silica gels provided reduced barriers to glucose diffusion, and the more aqueous microenvironment provided greater stability for the enzyme.

Duke Scholars

Published In

Sensors and actuators. B, Chemical

DOI

ISSN

0925-4005

Publication Date

November 2012

Volume

174

Start / End Page

373 / 379

Related Subject Headings

  • Analytical Chemistry
  • 4016 Materials engineering
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0912 Materials Engineering
  • 0301 Analytical Chemistry
  • 0205 Optical Physics
 

Citation

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Harris, J. M., Lopez, G. P., & Reichert, W. M. (2012). Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH. Sensors and Actuators. B, Chemical, 174, 373–379. https://doi.org/10.1016/j.snb.2012.08.046
Harris, James M., Gabriel P. Lopez, and William M. Reichert. “Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH.Sensors and Actuators. B, Chemical 174 (November 2012): 373–79. https://doi.org/10.1016/j.snb.2012.08.046.
Harris JM, Lopez GP, Reichert WM. Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH. Sensors and actuators B, Chemical. 2012 Nov;174:373–9.
Harris, James M., et al. “Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH.Sensors and Actuators. B, Chemical, vol. 174, Nov. 2012, pp. 373–79. Epmc, doi:10.1016/j.snb.2012.08.046.
Harris JM, Lopez GP, Reichert WM. Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH. Sensors and actuators B, Chemical. 2012 Nov;174:373–379.
Journal cover image

Published In

Sensors and actuators. B, Chemical

DOI

ISSN

0925-4005

Publication Date

November 2012

Volume

174

Start / End Page

373 / 379

Related Subject Headings

  • Analytical Chemistry
  • 4016 Materials engineering
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0912 Materials Engineering
  • 0301 Analytical Chemistry
  • 0205 Optical Physics