Prediction of feature spread for microarray printing using protein and DNA solutions

Printing experiments were conducted with a variety of DNA and protein samples for the optimization of microarray density and the correlation of feature size with the predictive model. The model performed well for all DNA solutions printed in 3xSSC buffer. The maximum microarray density was achieved on the GAPS substrate, with a predicted feature size of 145 μm, a recommended pitch of 155 μm, and a predicted density of 4800 features/cm2. The model also performed well for PBS buffer protein solutions printed on the GAPS slides. The maximum predictable microarray density was achieved in PBS buffer on the Telechem Super Amine substrate, with a predicted feature size of 150 μm, a recommended pitch of 160 μm, and a predicted density of 4500 features/cm2.

Duke Scholars

Author William M. Reichert Biomedical Engineering