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Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers.

Publication ,  Journal Article
Shin, BC; Wisniewski, N; Reichert, WM
Published in: Journal of biomaterials science. Polymer edition
January 2001

This study employed two water-soluble and nontoxic molecules, sucrose and glycerol, to enhance the permeability of PEG-PHEMA polymer gels coated onto 100 kDa molecular weight cutoff polyethersulfone (PES) microdialysis probes. Sucrose precoating of the probes prior to prepolymer coating prevented penetration of the prepolymer into the microdialysis membrane. Glycerol mixed with the prepolymer introduced porosity in the polymer coating upon curing. The sucrose and glycerol were completely removed by soaking in PBS after curing of the polymer coat on the probe tip. Polymer coated probe glucose permeability was tested by measuring glucose recovery from PBS solutions. Biocompatibility was assessed by measuring glucose recovery of polymer coated probes from heparanized whole porcine blood. Results show that the sucrose and glycerol treatments yielded polymer coated probes with glucose permeability nearly equal to bare probes when tested in PBS solution, but that this increased permeability was not observed when tested in whole blood. This suggests that the thickness of the polymer films (10-100 microm), while not a limiting factor in PBS solution, may have presented a diffusion barrier to glucose recovered from blood. Surprisingly, however, the polymer coated probes exhibited less thrombus formation that did the bare probes after blood exposure.

Duke Scholars

Published In

Journal of biomaterials science. Polymer edition

DOI

EISSN

1568-5624

ISSN

0920-5063

Publication Date

January 2001

Volume

12

Issue

5

Start / End Page

467 / 477

Related Subject Headings

  • Water
  • Swine
  • Sucrose
  • Solubility
  • Proteins
  • Polyhydroxyethyl Methacrylate
  • Polyethylene Glycols
  • Permeability
  • Microdialysis
  • Glycerol
 

Citation

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ICMJE
MLA
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Shin, B. C., Wisniewski, N., & Reichert, W. M. (2001). Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers. Journal of Biomaterials Science. Polymer Edition, 12(5), 467–477. https://doi.org/10.1163/156856201300194216
Shin, B. C., N. Wisniewski, and W. M. Reichert. “Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers.Journal of Biomaterials Science. Polymer Edition 12, no. 5 (January 2001): 467–77. https://doi.org/10.1163/156856201300194216.
Shin BC, Wisniewski N, Reichert WM. Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers. Journal of biomaterials science Polymer edition. 2001 Jan;12(5):467–77.
Shin, B. C., et al. “Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers.Journal of Biomaterials Science. Polymer Edition, vol. 12, no. 5, Jan. 2001, pp. 467–77. Epmc, doi:10.1163/156856201300194216.
Shin BC, Wisniewski N, Reichert WM. Water-soluble treatments to enhance glucose permeability of protein-resistant polymer overlayers. Journal of biomaterials science Polymer edition. 2001 Jan;12(5):467–477.

Published In

Journal of biomaterials science. Polymer edition

DOI

EISSN

1568-5624

ISSN

0920-5063

Publication Date

January 2001

Volume

12

Issue

5

Start / End Page

467 / 477

Related Subject Headings

  • Water
  • Swine
  • Sucrose
  • Solubility
  • Proteins
  • Polyhydroxyethyl Methacrylate
  • Polyethylene Glycols
  • Permeability
  • Microdialysis
  • Glycerol