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Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast.

Publication ,  Journal Article
Lippert, MJ; Freedman, JA; Barber, MA; Jinks-Robertson, S
Published in: Mol Cell Biol
June 2004

High levels of transcription are associated with increased mutation rates in Saccharomyces cerevisiae, a phenomenon termed transcription-associated mutation (TAM). To obtain insight into the mechanism of TAM, we obtained LYS2 forward mutation spectra under low- versus high-transcription conditions in which LYS2 was expressed from either the low-level pLYS2 promoter or the strong pGAL1-10 promoter, respectively. Because of the large size of the LYS2 locus, forward mutations first were mapped to specific LYS2 subregions, and then those mutations that occurred within a defined 736-bp target region were sequenced. In the low-transcription strain base substitutions comprised the majority (64%) of mutations, whereas short insertion-deletion mutations predominated (56%) in the high-transcription strain. Most notably, deletions of 2 nucleotides (nt) comprised 21% of the mutations in the high-transcription strain, and these events occurred predominantly at 5'-(G/C)AAA-3' sites. No -2 events were present in the low-transcription spectrum, thus identifying 2-nt deletions as a unique mutational signature for TAM.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

June 2004

Volume

24

Issue

11

Start / End Page

4801 / 4809

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Saccharomyces cerevisiae
  • Mutation
  • Molecular Sequence Data
  • Developmental Biology
  • Chromosome Mapping
  • Base Sequence
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Lippert, M. J., Freedman, J. A., Barber, M. A., & Jinks-Robertson, S. (2004). Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast. Mol Cell Biol, 24(11), 4801–4809. https://doi.org/10.1128/MCB.24.11.4801-4809.2004
Lippert, Malcolm J., Jennifer A. Freedman, Melissa A. Barber, and Sue Jinks-Robertson. “Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast.Mol Cell Biol 24, no. 11 (June 2004): 4801–9. https://doi.org/10.1128/MCB.24.11.4801-4809.2004.
Lippert MJ, Freedman JA, Barber MA, Jinks-Robertson S. Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast. Mol Cell Biol. 2004 Jun;24(11):4801–9.
Lippert, Malcolm J., et al. “Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast.Mol Cell Biol, vol. 24, no. 11, June 2004, pp. 4801–09. Pubmed, doi:10.1128/MCB.24.11.4801-4809.2004.
Lippert MJ, Freedman JA, Barber MA, Jinks-Robertson S. Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast. Mol Cell Biol. 2004 Jun;24(11):4801–4809.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

June 2004

Volume

24

Issue

11

Start / End Page

4801 / 4809

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Saccharomyces cerevisiae
  • Mutation
  • Molecular Sequence Data
  • Developmental Biology
  • Chromosome Mapping
  • Base Sequence
  • 11 Medical and Health Sciences
  • 06 Biological Sciences