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Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae.

Publication ,  Journal Article
Freedman, JA; Jinks-Robertson, S
Published in: Genetics
September 2002

The genetic requirements for spontaneous and transcription-stimulated mitotic recombination were determined using a recombination system that employs heterochromosomal lys2 substrates that can recombine only by crossover or only by gene conversion. The substrates were fused either to a constitutive low-level promoter (pLYS) or to a highly inducible promoter (pGAL). In the case of the "conversion-only" substrates the use of heterologous promoters allowed either the donor or the recipient allele to be highly transcribed. Transcription of the donor allele stimulated gene conversions in rad50, rad51, rad54, and rad59 mutants, but not in rad52, rad55, and rad57 mutants. In contrast, transcription of the recipient allele stimulated gene conversions in rad50, rad51, rad54, rad55, rad57, and rad59 mutants, but not in rad52 mutants. Finally, transcription stimulated crossovers in rad50, rad54, and rad59 mutants, but not in rad51, rad52, rad55, and rad57 mutants. These data are considered in relation to previously proposed molecular mechanisms of transcription-stimulated recombination and in relation to the roles of the recombination proteins.

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Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

September 2002

Volume

162

Issue

1

Start / End Page

15 / 27

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Rad52 DNA Repair and Recombination Protein
  • Plasmids
  • Mitosis
  • Gene Conversion
  • Developmental Biology
  • DNA-Binding Proteins
 

Citation

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ICMJE
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Freedman, J. A., & Jinks-Robertson, S. (2002). Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae. Genetics, 162(1), 15–27. https://doi.org/10.1093/genetics/162.1.15
Freedman, Jennifer A., and Sue Jinks-Robertson. “Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae.Genetics 162, no. 1 (September 2002): 15–27. https://doi.org/10.1093/genetics/162.1.15.
Freedman, Jennifer A., and Sue Jinks-Robertson. “Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae.Genetics, vol. 162, no. 1, Sept. 2002, pp. 15–27. Pubmed, doi:10.1093/genetics/162.1.15.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

September 2002

Volume

162

Issue

1

Start / End Page

15 / 27

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Rad52 DNA Repair and Recombination Protein
  • Plasmids
  • Mitosis
  • Gene Conversion
  • Developmental Biology
  • DNA-Binding Proteins