Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states
Publication
, Journal Article
Wozniak, M; Saunders, C; Schramm, N; Keefer, JR; Limbird, LE
Published in: Methods in Enzymology
2001
Epitope tagged α2-AR subtypes have been used to address a variety of cell biological questions, and the strategies used are readily applicable to all GPCR as well as other cell surface proteins. We have provided detailed protocols for successful utilization of the epitope-tagged receptor in the studies of protein localization and trafficking in epithelial cells, and the mechanisms by which this is achieved. We have also described reversible biotinytion strategies to examine agonist-dependent (and independent) receptor turnover at the cell surface.
Duke Scholars
Published In
Methods in Enzymology
DOI
ISSN
0076-6879
Publication Date
2001
Volume
343
Start / End Page
530 / 544
Related Subject Headings
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Wozniak, M., Saunders, C., Schramm, N., Keefer, J. R., & Limbird, L. E. (2001). Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states. Methods in Enzymology, 343, 530–544. https://doi.org/10.1016/S0076-6879(02)43156-4
Wozniak, M., C. Saunders, N. Schramm, J. R. Keefer, and L. E. Limbird. “Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states.” Methods in Enzymology 343 (2001): 530–44. https://doi.org/10.1016/S0076-6879(02)43156-4.
Wozniak M, Saunders C, Schramm N, Keefer JR, Limbird LE. Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states. Methods in Enzymology. 2001;343:530–44.
Wozniak, M., et al. “Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states.” Methods in Enzymology, vol. 343, 2001, pp. 530–44. Scival, doi:10.1016/S0076-6879(02)43156-4.
Wozniak M, Saunders C, Schramm N, Keefer JR, Limbird LE. Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states. Methods in Enzymology. 2001;343:530–544.
Published In
Methods in Enzymology
DOI
ISSN
0076-6879
Publication Date
2001
Volume
343
Start / End Page
530 / 544
Related Subject Headings
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology