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Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states

Publication ,  Journal Article
Wozniak, M; Saunders, C; Schramm, N; Keefer, JR; Limbird, LE
Published in: Methods in Enzymology
2001

Epitope tagged α2-AR subtypes have been used to address a variety of cell biological questions, and the strategies used are readily applicable to all GPCR as well as other cell surface proteins. We have provided detailed protocols for successful utilization of the epitope-tagged receptor in the studies of protein localization and trafficking in epithelial cells, and the mechanisms by which this is achieved. We have also described reversible biotinytion strategies to examine agonist-dependent (and independent) receptor turnover at the cell surface.

Duke Scholars

Published In

Methods in Enzymology

DOI

ISSN

0076-6879

Publication Date

2001

Volume

343

Start / End Page

530 / 544

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Wozniak, M., Saunders, C., Schramm, N., Keefer, J. R., & Limbird, L. E. (2001). Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states. Methods in Enzymology, 343, 530–544. https://doi.org/10.1016/S0076-6879(02)43156-4
Wozniak, M., C. Saunders, N. Schramm, J. R. Keefer, and L. E. Limbird. “Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states.” Methods in Enzymology 343 (2001): 530–44. https://doi.org/10.1016/S0076-6879(02)43156-4.
Wozniak, M., et al. “Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-naive and agonist-occupied states.” Methods in Enzymology, vol. 343, 2001, pp. 530–44. Scival, doi:10.1016/S0076-6879(02)43156-4.
Journal cover image

Published In

Methods in Enzymology

DOI

ISSN

0076-6879

Publication Date

2001

Volume

343

Start / End Page

530 / 544

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 0601 Biochemistry and Cell Biology