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Measurement of chloride movement in neuronal preparations.

Publication ,  Journal Article
Schwartz-Bloom, RD; Engblom, AC; Akerman, KE; Inglefield, JR
Published in: Curr Protoc Neurosci
May 2001

In this unit, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand-gated chloride movement in vesicular structures called synaptoneurosomes (also referred to as microsacs), in cultured neurons, and in the acute brain slice. These techniques can be applied to other ions as well. The measurement of uptake and efflux of radioisotopic chloride in synaptoneurosomes is used to study the responses of gamma-aminobutyric acid (GABA) receptors, which are coupled to chloride channels. Similar chloride flux assays for primary neuronal cultures are also presented. Alternatively, the efflux of chloride from synaptoneurosomes and primary neuronal cultures can be studied using fluorescent dyes and photometry. Finally, the measurement of chloride uptake can be studied in individual neurons in brain slices using fluorescent dyes and optical imaging by nonconfocal and confocal microscopy. Several support protocols are provided as well, outlining the preparation of synaptoneurosomes from specific brain regions, and the preparation, loading, and calibration of chloride-sensitive fluorescent dyes.

Duke Scholars

Published In

Curr Protoc Neurosci

DOI

EISSN

1934-8576

Publication Date

May 2001

Volume

Chapter 7

Start / End Page

Unit7.10

Location

United States

Related Subject Headings

  • Synaptosomes
  • Receptors, GABA
  • Rats
  • Radioisotopes
  • Quinolinium Compounds
  • Neurons
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Mice
  • Ion Channel Gating
 

Citation

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Schwartz-Bloom, R. D., Engblom, A. C., Akerman, K. E., & Inglefield, J. R. (2001). Measurement of chloride movement in neuronal preparations. Curr Protoc Neurosci, Chapter 7, Unit7.10. https://doi.org/10.1002/0471142301.ns0710s04
Schwartz-Bloom, R. D., A. C. Engblom, K. E. Akerman, and J. R. Inglefield. “Measurement of chloride movement in neuronal preparations.Curr Protoc Neurosci Chapter 7 (May 2001): Unit7.10. https://doi.org/10.1002/0471142301.ns0710s04.
Schwartz-Bloom RD, Engblom AC, Akerman KE, Inglefield JR. Measurement of chloride movement in neuronal preparations. Curr Protoc Neurosci. 2001 May;Chapter 7:Unit7.10.
Schwartz-Bloom, R. D., et al. “Measurement of chloride movement in neuronal preparations.Curr Protoc Neurosci, vol. Chapter 7, May 2001, p. Unit7.10. Pubmed, doi:10.1002/0471142301.ns0710s04.
Schwartz-Bloom RD, Engblom AC, Akerman KE, Inglefield JR. Measurement of chloride movement in neuronal preparations. Curr Protoc Neurosci. 2001 May;Chapter 7:Unit7.10.

Published In

Curr Protoc Neurosci

DOI

EISSN

1934-8576

Publication Date

May 2001

Volume

Chapter 7

Start / End Page

Unit7.10

Location

United States

Related Subject Headings

  • Synaptosomes
  • Receptors, GABA
  • Rats
  • Radioisotopes
  • Quinolinium Compounds
  • Neurons
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Mice
  • Ion Channel Gating