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Fiber optic SERS-based plasmonics nanobiosensing in single living cells

Publication ,  Journal Article
Scaffidi, JP; Gregas, MK; Seewaldt, V; Dinh, TV
Published in: Proceedings of SPIE - The International Society for Optical Engineering
September 8, 2009

We describe the development of small molecule-sensitive plasmonics-active fiber-optic nanoprobes suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The practical utility of SERS-based fiber-optic nanoprobes is illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor biologically-relevant small molecules at the single cell level. ©2009 SPIE.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

September 8, 2009

Volume

7312

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Scaffidi, J. P., Gregas, M. K., Seewaldt, V., & Dinh, T. V. (2009). Fiber optic SERS-based plasmonics nanobiosensing in single living cells. Proceedings of SPIE - The International Society for Optical Engineering, 7312. https://doi.org/10.1117/12.824453
Scaffidi, J. P., M. K. Gregas, V. Seewaldt, and T. V. Dinh. “Fiber optic SERS-based plasmonics nanobiosensing in single living cells.” Proceedings of SPIE - The International Society for Optical Engineering 7312 (September 8, 2009). https://doi.org/10.1117/12.824453.
Scaffidi JP, Gregas MK, Seewaldt V, Dinh TV. Fiber optic SERS-based plasmonics nanobiosensing in single living cells. Proceedings of SPIE - The International Society for Optical Engineering. 2009 Sep 8;7312.
Scaffidi, J. P., et al. “Fiber optic SERS-based plasmonics nanobiosensing in single living cells.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 7312, Sept. 2009. Scopus, doi:10.1117/12.824453.
Scaffidi JP, Gregas MK, Seewaldt V, Dinh TV. Fiber optic SERS-based plasmonics nanobiosensing in single living cells. Proceedings of SPIE - The International Society for Optical Engineering. 2009 Sep 8;7312.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

September 8, 2009

Volume

7312

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering