Skip to main content
Journal cover image

Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3.

Publication ,  Journal Article
Barr, AJ; Marjoram, R; Xu, J; Snyderman, R
Published in: Biochem Biophys Res Commun
April 26, 2002

Phospholipase C (PLC)-beta enzymes (isoenzymes beta 1-beta 4) are activated by G protein subunits, leading to the generation of intracellular messengers which mobilize calcium and activate protein kinase C. It has recently been recognized that these enzymes interact with and are regulated by proteins other than G proteins. Using the yeast two-hybrid technique to screen a leukocyte library we identified mitogen-activated protein kinase kinase 3 (MKK3) as a partner of PLC-beta 2. The interaction was confirmed by co-immunoprecipitation assays which indicated that MKK3 interacts with PLC-beta 2, but not with other PLC-betas. PLC-beta 2 interacted weakly with MKK6, which is related to MKK3, but not with the other MKK3 tested. The region of PLC-beta 2 involved in the interaction with MKK3 was mapped to the C-terminus of PLC-beta 2. p38MAPK also co-immunoprecipitated with PLC-beta 2. The data suggest that PLC-beta 2 serves an unappreciated role assembling components of the p38MAPK signaling module.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

April 26, 2002

Volume

293

Issue

1

Start / End Page

647 / 652

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Type C Phospholipases
  • Saccharomyces cerevisiae
  • Protein-Tyrosine Kinases
  • Phospholipase C beta
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • MAP Kinase Kinase 3
  • Leukocytes
  • Isoenzymes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Barr, A. J., Marjoram, R., Xu, J., & Snyderman, R. (2002). Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3. Biochem Biophys Res Commun, 293(1), 647–652. https://doi.org/10.1016/S0006-291X(02)00259-0
Barr, Alastair J., Robin Marjoram, Jing Xu, and Ralph Snyderman. “Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3.Biochem Biophys Res Commun 293, no. 1 (April 26, 2002): 647–52. https://doi.org/10.1016/S0006-291X(02)00259-0.
Barr AJ, Marjoram R, Xu J, Snyderman R. Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3. Biochem Biophys Res Commun. 2002 Apr 26;293(1):647–52.
Barr, Alastair J., et al. “Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3.Biochem Biophys Res Commun, vol. 293, no. 1, Apr. 2002, pp. 647–52. Pubmed, doi:10.1016/S0006-291X(02)00259-0.
Barr AJ, Marjoram R, Xu J, Snyderman R. Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3. Biochem Biophys Res Commun. 2002 Apr 26;293(1):647–652.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

April 26, 2002

Volume

293

Issue

1

Start / End Page

647 / 652

Location

United States

Related Subject Headings

  • p38 Mitogen-Activated Protein Kinases
  • Type C Phospholipases
  • Saccharomyces cerevisiae
  • Protein-Tyrosine Kinases
  • Phospholipase C beta
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • MAP Kinase Kinase 3
  • Leukocytes
  • Isoenzymes