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Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins.

Publication ,  Journal Article
Polakis, PG; Evans, T; Snyderman, R
Published in: Biochem Biophys Res Commun
May 30, 1989

The radiolabeled formylpeptide chemoattractant receptor, partially purified by wheat germ agglutinin-Sepharose chromatography, eluted in three distinct peaks when chromatographed on DEAE-Fractogel. Incubation of the lectin-Sepharose purified receptor with 100 microM guanosine 5-(3-O-thio) triphosphate markedly altered the distribution of the radiolabeled receptor when chromatographed on the ion exchange resin. Peaks 2 and 3 were reduced by approximately 50% while peak 1 was concomitantly increased. Western blot analysis revealed the presence of a 40 kDa GTP-binding protein alpha-subunit only in peak 3. Incubation of Western blots with [alpha-32P]GTP detected low molecular mass GTP-binding proteins (24 and 26 kDa) that coeluted with the receptor in peak 2. Incubation of the peak 1 receptor fractions with fractions containing a mixture of GTP binding proteins resulted in the generation of peaks 2 and 3 when chromatographed on DEAE-Fractogel. These results demonstrate that the chromatographic behavior of the formylpeptide receptor is dependent upon its association with GTP binding proteins and that more than one type of GTP-binding protein may be involved.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 30, 1989

Volume

161

Issue

1

Start / End Page

276 / 283

Location

United States

Related Subject Headings

  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Radioligand Assay
  • N-Formylmethionine Leucyl-Phenylalanine
  • Membrane Proteins
  • Immunoblotting
  • Humans
  • GTP-Binding Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Chromatography, Ion Exchange
 

Citation

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MLA
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Polakis, P. G., Evans, T., & Snyderman, R. (1989). Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins. Biochem Biophys Res Commun, 161(1), 276–283. https://doi.org/10.1016/0006-291x(89)91592-1
Polakis, P. G., T. Evans, and R. Snyderman. “Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins.Biochem Biophys Res Commun 161, no. 1 (May 30, 1989): 276–83. https://doi.org/10.1016/0006-291x(89)91592-1.
Polakis PG, Evans T, Snyderman R. Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins. Biochem Biophys Res Commun. 1989 May 30;161(1):276–83.
Polakis, P. G., et al. “Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins.Biochem Biophys Res Commun, vol. 161, no. 1, May 1989, pp. 276–83. Pubmed, doi:10.1016/0006-291x(89)91592-1.
Polakis PG, Evans T, Snyderman R. Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteins. Biochem Biophys Res Commun. 1989 May 30;161(1):276–283.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 30, 1989

Volume

161

Issue

1

Start / End Page

276 / 283

Location

United States

Related Subject Headings

  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Radioligand Assay
  • N-Formylmethionine Leucyl-Phenylalanine
  • Membrane Proteins
  • Immunoblotting
  • Humans
  • GTP-Binding Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Chromatography, Ion Exchange