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The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila.

Publication ,  Journal Article
Spana, EP; Doe, CQ
Published in: Development (Cambridge, England)
October 1995

Both intrinsic and extrinsic factors are known to regulate sibling cell fate. Here we describe a novel mechanism for the asymmetric localization of a transcription factor to one daughter cell at mitosis. The Drosophila CNS develops from asymmetrically dividing neuroblasts, which give rise to a large neuroblast and a smaller ganglion mother cell (GMC). The prospero gene encodes a transcription factor necessary for proper GMC gene expression. We show that the prospero protein is synthesized in the neuroblast where it is localized to the F-actin cell cortex. At mitosis, prospero is asymmetrically localized to the budding GMC and excluded from the neuroblast. After cytokinesis, prospero is translocated from the GMC cortex into the nucleus. Asymmetric cortical localization of prospero in neuroblasts requires entry into mitosis; it does not depend on numb function. prospero is also observed in cortical crescents in dividing precursors of the peripheral nervous system and adult midgut. The asymmetric cortical localization of prospero at mitosis is a mechanism for rapidly establishing distinct sibling cell fates in the CNS and possibly other tissues.

Duke Scholars

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

October 1995

Volume

121

Issue

10

Start / End Page

3187 / 3195

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Peripheral Nervous System
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Mitosis
  • Microscopy, Confocal
  • Intestines
  • Histocytochemistry
  • Gene Expression
 

Citation

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Spana, E. P., & Doe, C. Q. (1995). The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila. Development (Cambridge, England), 121(10), 3187–3195. https://doi.org/10.1242/dev.121.10.3187
Spana, E. P., and C. Q. Doe. “The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila.Development (Cambridge, England) 121, no. 10 (October 1995): 3187–95. https://doi.org/10.1242/dev.121.10.3187.
Spana, E. P., and C. Q. Doe. “The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila.Development (Cambridge, England), vol. 121, no. 10, Oct. 1995, pp. 3187–95. Epmc, doi:10.1242/dev.121.10.3187.
Spana EP, Doe CQ. The prospero transcription factor is asymmetrically localized to the cell cortex during neuroblast mitosis in Drosophila. Development (Cambridge, England). 1995 Oct;121(10):3187–3195.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

October 1995

Volume

121

Issue

10

Start / End Page

3187 / 3195

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Peripheral Nervous System
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Mitosis
  • Microscopy, Confocal
  • Intestines
  • Histocytochemistry
  • Gene Expression