Skip to main content

Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA with adjuvant monophosphoryl lipid A protects swine from a homologous but not a heterologous challenge.

Publication ,  Journal Article
Fusco, WG; Afonina, G; Nepluev, I; Cholon, DM; Choudhary, N; Routh, PA; Almond, GW; Orndorff, PE; Staats, H; Hobbs, MM; Leduc, I; Elkins, C
Published in: Infect Immun
September 2010

Haemophilus ducreyi, the etiological agent of chancroid, has a strict requirement for heme, which it acquires from its only natural host, humans. Previously, we showed that a vaccine preparation containing the native hemoglobin receptor HgbA purified from H. ducreyi class I strain 35000HP (nHgbAI) and administered with Freund's adjuvant provided complete protection against a homologous challenge. In the current study, we investigated whether nHgbAI dispensed with monophosphoryl lipid A (MPL), an adjuvant approved for use in humans, offered protection against a challenge with H. ducreyi strain 35000HP expressing either class I or class II HgbA (35000HPhgbAI and 35000HPhgbAII, respectively). Pigs immunized with the nHgbAI/MPL vaccine were protected against a challenge from homologous H. ducreyi strain 35000HPhgbAI but not heterologous strain 35000HPhgbAII, as evidenced by the isolation of only strain 35000HPhgbAII from nHgbAI-immunized pigs. Furthermore, histological analysis of the lesions showed striking differences between mock-immunized and nHgbAI-immunized animals challenged with strains 35000HPhgbAI but not those challenged with strain 35000HPhgbAII. Mock-immunized pigs were not protected from a challenge by either strain. The enzyme-linked immunosorbent assay (ELISA) activity of the nHgbAI/MPL antiserum was lower than the activity of antiserum from animals immunized with the nHgbAI/Freund's vaccine; however, anti-nHgbAI from both studies bound whole cells of 35000HPhgbAI better than 35000HPhgbAII and partially blocked hemoglobin binding to nHgbAI. In conclusion, despite eliciting lower antibody ELISA activity than the nHgbAI/Freund's, the nHgbAI/MPL vaccine provided protection against a challenge with homologous but not heterologous H. ducreyi, suggesting that a bivalent HgbA vaccine may be needed.

Duke Scholars

Published In

Infect Immun

DOI

EISSN

1098-5522

Publication Date

September 2010

Volume

78

Issue

9

Start / End Page

3763 / 3772

Location

United States

Related Subject Headings

  • Swine
  • Microbiology
  • Lipid A
  • Immunization
  • Immune Sera
  • Haemophilus ducreyi
  • Enzyme-Linked Immunosorbent Assay
  • Chancroid
  • Carrier Proteins
  • Bacterial Vaccines
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fusco, W. G., Afonina, G., Nepluev, I., Cholon, D. M., Choudhary, N., Routh, P. A., … Elkins, C. (2010). Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA with adjuvant monophosphoryl lipid A protects swine from a homologous but not a heterologous challenge. Infect Immun, 78(9), 3763–3772. https://doi.org/10.1128/IAI.00217-10
Fusco, William G., Galyna Afonina, Igor Nepluev, Deborah M. Cholon, Neelima Choudhary, Patricia A. Routh, Glenn W. Almond, et al. “Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA with adjuvant monophosphoryl lipid A protects swine from a homologous but not a heterologous challenge.Infect Immun 78, no. 9 (September 2010): 3763–72. https://doi.org/10.1128/IAI.00217-10.
Fusco, William G., et al. “Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA with adjuvant monophosphoryl lipid A protects swine from a homologous but not a heterologous challenge.Infect Immun, vol. 78, no. 9, Sept. 2010, pp. 3763–72. Pubmed, doi:10.1128/IAI.00217-10.
Fusco WG, Afonina G, Nepluev I, Cholon DM, Choudhary N, Routh PA, Almond GW, Orndorff PE, Staats H, Hobbs MM, Leduc I, Elkins C. Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA with adjuvant monophosphoryl lipid A protects swine from a homologous but not a heterologous challenge. Infect Immun. 2010 Sep;78(9):3763–3772.

Published In

Infect Immun

DOI

EISSN

1098-5522

Publication Date

September 2010

Volume

78

Issue

9

Start / End Page

3763 / 3772

Location

United States

Related Subject Headings

  • Swine
  • Microbiology
  • Lipid A
  • Immunization
  • Immune Sera
  • Haemophilus ducreyi
  • Enzyme-Linked Immunosorbent Assay
  • Chancroid
  • Carrier Proteins
  • Bacterial Vaccines