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Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion.

Publication ,  Journal Article
Stamer, WD; Seftor, RE; Williams, SK; Samaha, HA; Snyder, RW
Published in: Curr Eye Res
July 1995

Like corneal endothelial cells, human trabecular meshwork cells are believed to be of neural crest origin, but demonstrate physiological properties and an antithrombogenic surface similar to vascular endothelial cells. One current method for isolating trabecular meshwork cells utilizes the motile nature of these cells to migrate away from a trabecular meshwork explant in culture to more distal regions of the culture dish. This 'outgrowth' technique is limited in practice by the relatively small number of cells that migrate per explant per unit time, thus hindering the ability to gather sufficient numbers of cells for comprehensive experimentation. For this reason, we have modified an extracellular matrix digestion technique in current use for the isolation of microvascular endothelial cells to isolate human trabecular meshwork cells. This procedure is both efficient and rapid for isolating large numbers of trabecular meshwork cells and results in the availability of trabecular meshwork cells in sufficient quantities for subsequent experimentation.

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Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

July 1995

Volume

14

Issue

7

Start / End Page

611 / 617

Location

England

Related Subject Headings

  • Trabecular Meshwork
  • Tissue Plasminogen Activator
  • Ophthalmology & Optometry
  • Middle Aged
  • Infant, Newborn
  • Infant
  • Immunoenzyme Techniques
  • Humans
  • Extracellular Matrix
  • Collagenases
 

Citation

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Stamer, W. D., Seftor, R. E., Williams, S. K., Samaha, H. A., & Snyder, R. W. (1995). Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion. Curr Eye Res, 14(7), 611–617. https://doi.org/10.3109/02713689508998409
Stamer, W. D., R. E. Seftor, S. K. Williams, H. A. Samaha, and R. W. Snyder. “Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion.Curr Eye Res 14, no. 7 (July 1995): 611–17. https://doi.org/10.3109/02713689508998409.
Stamer WD, Seftor RE, Williams SK, Samaha HA, Snyder RW. Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion. Curr Eye Res. 1995 Jul;14(7):611–7.
Stamer, W. D., et al. “Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion.Curr Eye Res, vol. 14, no. 7, July 1995, pp. 611–17. Pubmed, doi:10.3109/02713689508998409.
Stamer WD, Seftor RE, Williams SK, Samaha HA, Snyder RW. Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion. Curr Eye Res. 1995 Jul;14(7):611–617.
Journal cover image

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

July 1995

Volume

14

Issue

7

Start / End Page

611 / 617

Location

England

Related Subject Headings

  • Trabecular Meshwork
  • Tissue Plasminogen Activator
  • Ophthalmology & Optometry
  • Middle Aged
  • Infant, Newborn
  • Infant
  • Immunoenzyme Techniques
  • Humans
  • Extracellular Matrix
  • Collagenases