Skip to main content

Calcium binding by monosulfate esters of taurochenodeoxycholate.

Publication ,  Journal Article
Stevens, RD; Lack, L; Killenberg, PG
Published in: J Lipid Res
April 1991

The effect of sulfate esterification of the 3 alpha- or 7 alpha-hydroxyl groups of taurochenodeoxycholate on calcium binding was studied at 0.154 M NaCl in the presence and absence of phosphatidylcholine using a calcium electrode. For comparison, similar studies were made with taurochenodeoxycholate, taurodeoxycholate, and taurocholate. No high affinity calcium binding was demonstrable for any of these bile salts in pre-micellar solutions. Taurine-conjugated bile salts have greater affinity for calcium when in a micellar form. At elevated bile salt concentrations, the calcium binding of unsulfated dihydroxy taurine conjugates was similar to that of the monosulfate esters of taurochenodeoxycholate. The presence of phosphatidylcholine decreased calcium binding of the unsulfated dihydroxy bile salts and slightly increased calcium binding by taurocholate. However, the addition of phosphatidylcholine to monosulfate esters of taurochenodeoxycholate results in large increments in calcium binding. The results indicate that increased calcium binding due to the presence of phosphatidylcholine in bile salt solutions depends, in part, on the hydrophilicity of the bile salt and that the interaction of monosulfate esters of taurochenodeoxycholate with phosphatidylcholine leads to the formation of a high affinity calcium binding site.

Duke Scholars

Published In

J Lipid Res

ISSN

0022-2275

Publication Date

April 1991

Volume

32

Issue

4

Start / End Page

621 / 627

Location

United States

Related Subject Headings

  • Taurodeoxycholic Acid
  • Taurocholic Acid
  • Taurochenodeoxycholic Acid
  • Sulfates
  • Phosphatidylcholines
  • Esterification
  • Calcium
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Bile Acids and Salts
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stevens, R. D., Lack, L., & Killenberg, P. G. (1991). Calcium binding by monosulfate esters of taurochenodeoxycholate. J Lipid Res, 32(4), 621–627.
Stevens, R. D., L. Lack, and P. G. Killenberg. “Calcium binding by monosulfate esters of taurochenodeoxycholate.J Lipid Res 32, no. 4 (April 1991): 621–27.
Stevens RD, Lack L, Killenberg PG. Calcium binding by monosulfate esters of taurochenodeoxycholate. J Lipid Res. 1991 Apr;32(4):621–7.
Stevens, R. D., et al. “Calcium binding by monosulfate esters of taurochenodeoxycholate.J Lipid Res, vol. 32, no. 4, Apr. 1991, pp. 621–27.
Stevens RD, Lack L, Killenberg PG. Calcium binding by monosulfate esters of taurochenodeoxycholate. J Lipid Res. 1991 Apr;32(4):621–627.

Published In

J Lipid Res

ISSN

0022-2275

Publication Date

April 1991

Volume

32

Issue

4

Start / End Page

621 / 627

Location

United States

Related Subject Headings

  • Taurodeoxycholic Acid
  • Taurocholic Acid
  • Taurochenodeoxycholic Acid
  • Sulfates
  • Phosphatidylcholines
  • Esterification
  • Calcium
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Bile Acids and Salts