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Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium.

Publication ,  Journal Article
Anderson, WW; Stasheff, SF; Swartzwelder, HS; Wilson, WA
Published in: Brain Res
November 5, 1990

All-or-none electrographic seizures (EGSs) were studied in hippocampal slices from young (21- to 38-day-old) rats in medium containing low (0 mM) or physiological (0.9 mM) levels of magnesium, with and without the GABAB agonist baclofen. Extracellular recording and stimulation were performed in stratum pyramidale and stratum radiatum of CA3, respectively. EGS activity was induced by exposure to low-Mg medium or by delivering repetitive stimulus trains in physiological Mg medium. After EGS activity had stabilized, the EGSs were tested for all-or-none behavior by varying the number of pulses in a train. An EGS was considered all-or-none if subthreshold stimulation produced no afterdischarge bursts, and if the EGS duration was largely independent of the number of suprathreshold stimulus pulses. According to this measure, EGSs in Mg-free + baclofen medium were all-or-none. EGSs evoked in physiological Mg medium were also all-or-none, although the threshold was higher, and the EGS duration lower, than in Mg-free medium. This all-or-none characteristic was observed whether the EGSs were induced by prior exposure to Mg-free medium or by repetitive stimulation, and in the presence and absence of baclofen. The all-or-none characteristic suggests that while the triggering mechanism for EGSs is strongly dependent on stimulus intensity, regenerative mechanisms--independent of stimulus intensity--are responsible for the maintenance of EGSs. EGSs are also terminated by mechanisms not dependent on stimulus intensity.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

November 5, 1990

Volume

532

Issue

1-2

Start / End Page

288 / 298

Location

Netherlands

Related Subject Headings

  • Seizures
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Magnesium
  • In Vitro Techniques
  • Hippocampus
  • Electric Stimulation
  • Culture Media
 

Citation

APA
Chicago
ICMJE
MLA
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Anderson, W. W., Stasheff, S. F., Swartzwelder, H. S., & Wilson, W. A. (1990). Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium. Brain Res, 532(1–2), 288–298. https://doi.org/10.1016/0006-8993(90)91771-8
Anderson, W. W., S. F. Stasheff, H. S. Swartzwelder, and W. A. Wilson. “Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium.Brain Res 532, no. 1–2 (November 5, 1990): 288–98. https://doi.org/10.1016/0006-8993(90)91771-8.
Anderson WW, Stasheff SF, Swartzwelder HS, Wilson WA. Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium. Brain Res. 1990 Nov 5;532(1–2):288–98.
Anderson, W. W., et al. “Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium.Brain Res, vol. 532, no. 1–2, Nov. 1990, pp. 288–98. Pubmed, doi:10.1016/0006-8993(90)91771-8.
Anderson WW, Stasheff SF, Swartzwelder HS, Wilson WA. Regenerative, all-or-none electrographic seizures in the rat hippocampal slice in Mg-free and physiological medium. Brain Res. 1990 Nov 5;532(1–2):288–298.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

November 5, 1990

Volume

532

Issue

1-2

Start / End Page

288 / 298

Location

Netherlands

Related Subject Headings

  • Seizures
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Magnesium
  • In Vitro Techniques
  • Hippocampus
  • Electric Stimulation
  • Culture Media