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Sensing membrane stress with near IR-emissive porphyrins.

Publication ,  Journal Article
Kamat, NP; Liao, Z; Moses, LE; Rawson, J; Therien, MJ; Dmochowski, IJ; Hammer, DA
Published in: Proceedings of the National Academy of Sciences of the United States of America
August 2011

Probes embedded within a structure can enable prediction of material behavior or failure. Carefully assembled composites that respond intelligently to physical changes within a material could be useful as intrinsic sensors. Molecular rotors are one such tool that can respond optically to physical environmental changes. Here, we propose to use molecular rotors within a polymersome membrane to report membrane stress. Using supermolecular porphyrin-based fluorophores as rotors, we characterize changes in the optical emission of these near-infrared (NIR) emissive probes embedded within the hydrophobic core of the polymersome membrane. The configuration of entrapped fluorophore depends on the available space within the membrane; in response to increased volume, emission is blue shifted. We used this feature to study how shifts in fluorescence correlate to membrane integrity, imparted by membrane stress. We monitored changes in emission of these porphyrin-based fluorophores resulting from membrane stress produced through a range of physical and chemical perturbations, including surfactant-induced lysis, hydrolytic lysis, thermal degradation, and applied stress by micropipette aspiration. This paper comprehensively illustrates the potential for supermolecular porphyrin-based fluorophores to detect intrinsic physical changes in a wide variety of environments, and suggests how molecular rotors may be used in soft materials science and biology as sensors.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

August 2011

Volume

108

Issue

34

Start / End Page

13984 / 13989

Related Subject Headings

  • Stress, Physiological
  • Spectrometry, Fluorescence
  • Porphyrins
  • Membranes, Artificial
  • Infrared Rays
  • Fluorescent Dyes
 

Citation

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Kamat, N. P., Liao, Z., Moses, L. E., Rawson, J., Therien, M. J., Dmochowski, I. J., & Hammer, D. A. (2011). Sensing membrane stress with near IR-emissive porphyrins. Proceedings of the National Academy of Sciences of the United States of America, 108(34), 13984–13989. https://doi.org/10.1073/pnas.1102125108
Kamat, Neha P., Zhengzheng Liao, Laurel E. Moses, Jeff Rawson, Michael J. Therien, Ivan J. Dmochowski, and Daniel A. Hammer. “Sensing membrane stress with near IR-emissive porphyrins.Proceedings of the National Academy of Sciences of the United States of America 108, no. 34 (August 2011): 13984–89. https://doi.org/10.1073/pnas.1102125108.
Kamat NP, Liao Z, Moses LE, Rawson J, Therien MJ, Dmochowski IJ, et al. Sensing membrane stress with near IR-emissive porphyrins. Proceedings of the National Academy of Sciences of the United States of America. 2011 Aug;108(34):13984–9.
Kamat, Neha P., et al. “Sensing membrane stress with near IR-emissive porphyrins.Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 34, Aug. 2011, pp. 13984–89. Epmc, doi:10.1073/pnas.1102125108.
Kamat NP, Liao Z, Moses LE, Rawson J, Therien MJ, Dmochowski IJ, Hammer DA. Sensing membrane stress with near IR-emissive porphyrins. Proceedings of the National Academy of Sciences of the United States of America. 2011 Aug;108(34):13984–13989.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

August 2011

Volume

108

Issue

34

Start / End Page

13984 / 13989

Related Subject Headings

  • Stress, Physiological
  • Spectrometry, Fluorescence
  • Porphyrins
  • Membranes, Artificial
  • Infrared Rays
  • Fluorescent Dyes