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Separation and quantitation of serum constituents associated with calcium by gel filtration.

Publication ,  Journal Article
Toffaletti, J; Gitelman, HJ; Savory, J
Published in: Clin Chem
December 1976

Gel filtration of serum by use of polyacrylamide beads (Biogel P-2) separates total colcium into four distinct peaks: an initial peak, corresponding to protein-bound calcium; a second peak containing the calcium complexes of citrate, phosphate, lactate, and sulfate; a third peak containing calcium bicarbonate; and a fourth peak or trough corresponding to the serum ionic calcium. An eluent containing (per liter) 140 mmol of sodium, 1.1o mmol of calcium, 0.50 mmol of magnesium, and 10 mmol of 2-([tris(hydroxymethyl)methyl]amino)ethanesulfonic acid(pH 7.40 at 37 degrees C) provides physiological conditions that determine the equilibria between these calcium components. Association constants determined under these conditions permit calculation of the expected concentration of the calcium complexes in each tube of eluent, and these concentrations closely correspond to the amount of bound calcium measured experimentally. The mean distribution of calcium in healthy individuals, as determined by this method, is (per liter): calcium protein, 1.00 mmol; calcium complexes, 0.31 mmol; and ionic calcium, 1.07 mmol.

Duke Scholars

Published In

Clin Chem

ISSN

0009-9147

Publication Date

December 1976

Volume

22

Issue

12

Start / End Page

1968 / 1972

Location

England

Related Subject Headings

  • Sulfates
  • Salts
  • Phosphates
  • Lactates
  • Kinetics
  • Humans
  • General Clinical Medicine
  • Citrates
  • Chromatography, Gel
  • Carrier Proteins
 

Citation

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Toffaletti, J., Gitelman, H. J., & Savory, J. (1976). Separation and quantitation of serum constituents associated with calcium by gel filtration. Clin Chem, 22(12), 1968–1972.
Toffaletti, J., H. J. Gitelman, and J. Savory. “Separation and quantitation of serum constituents associated with calcium by gel filtration.Clin Chem 22, no. 12 (December 1976): 1968–72.
Toffaletti J, Gitelman HJ, Savory J. Separation and quantitation of serum constituents associated with calcium by gel filtration. Clin Chem. 1976 Dec;22(12):1968–72.
Toffaletti, J., et al. “Separation and quantitation of serum constituents associated with calcium by gel filtration.Clin Chem, vol. 22, no. 12, Dec. 1976, pp. 1968–72.
Toffaletti J, Gitelman HJ, Savory J. Separation and quantitation of serum constituents associated with calcium by gel filtration. Clin Chem. 1976 Dec;22(12):1968–1972.

Published In

Clin Chem

ISSN

0009-9147

Publication Date

December 1976

Volume

22

Issue

12

Start / End Page

1968 / 1972

Location

England

Related Subject Headings

  • Sulfates
  • Salts
  • Phosphates
  • Lactates
  • Kinetics
  • Humans
  • General Clinical Medicine
  • Citrates
  • Chromatography, Gel
  • Carrier Proteins