Skip to main content
Journal cover image

Combining topographical and genetic cues to promote neuronal fate specification in stem cells.

Publication ,  Journal Article
Purcell, EK; Naim, Y; Yang, A; Leach, MK; Velkey, JM; Duncan, RK; Corey, JM
Published in: Biomacromolecules
November 12, 2012

There is little remedy for the devastating effects resulting from neuronal loss caused by neural injury or neurodegenerative disease. Reconstruction of damaged neural circuitry with stem cell-derived neurons is a promising approach to repair these defects, but controlling differentiation and guiding synaptic integration with existing neurons remain significant unmet challenges. Biomaterial surfaces can present nanoscale topographical cues that influence neuronal differentiation and process outgrowth. By combining these scaffolds with additional molecular biology strategies, synergistic control over cell fate can be achieved. Here, we review recent progress in promoting neuronal fate using techniques at the interface of biomaterial science and genetic engineering. New data demonstrates that combining nanofiber topography with an induced genetic program enhances neuritogenesis in a synergistic fashion. We propose combining patterned biomaterial surface cues with prescribed genetic programs to achieve neuronal cell fates with the desired sublineage specification, neurochemical profile, targeted integration, and electrophysiological properties.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biomacromolecules

DOI

EISSN

1526-4602

Publication Date

November 12, 2012

Volume

13

Issue

11

Start / End Page

3427 / 3438

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polymers
  • Neurons
  • Neurogenesis
  • Neurodegenerative Diseases
  • Nerve Regeneration
  • Genetic Engineering
  • Gene Transfer Techniques
  • Embryonic Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Purcell, E. K., Naim, Y., Yang, A., Leach, M. K., Velkey, J. M., Duncan, R. K., & Corey, J. M. (2012). Combining topographical and genetic cues to promote neuronal fate specification in stem cells. Biomacromolecules, 13(11), 3427–3438. https://doi.org/10.1021/bm301220k
Purcell, Erin K., Youssef Naim, Amy Yang, Michelle K. Leach, J Matthew Velkey, R Keith Duncan, and Joseph M. Corey. “Combining topographical and genetic cues to promote neuronal fate specification in stem cells.Biomacromolecules 13, no. 11 (November 12, 2012): 3427–38. https://doi.org/10.1021/bm301220k.
Purcell EK, Naim Y, Yang A, Leach MK, Velkey JM, Duncan RK, et al. Combining topographical and genetic cues to promote neuronal fate specification in stem cells. Biomacromolecules. 2012 Nov 12;13(11):3427–38.
Purcell, Erin K., et al. “Combining topographical and genetic cues to promote neuronal fate specification in stem cells.Biomacromolecules, vol. 13, no. 11, Nov. 2012, pp. 3427–38. Pubmed, doi:10.1021/bm301220k.
Purcell EK, Naim Y, Yang A, Leach MK, Velkey JM, Duncan RK, Corey JM. Combining topographical and genetic cues to promote neuronal fate specification in stem cells. Biomacromolecules. 2012 Nov 12;13(11):3427–3438.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

Publication Date

November 12, 2012

Volume

13

Issue

11

Start / End Page

3427 / 3438

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Polymers
  • Neurons
  • Neurogenesis
  • Neurodegenerative Diseases
  • Nerve Regeneration
  • Genetic Engineering
  • Gene Transfer Techniques
  • Embryonic Stem Cells