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Plasticity in the adult human oligodendrocyte lineage.

Publication ,  Journal Article
Gogate, N; Verma, L; Zhou, JM; Milward, E; Rusten, R; O'Connor, M; Kufta, C; Kim, J; Hudson, L; Dubois-Dalcq, M
Published in: J Neurosci
August 1994

Preoligodendrocytes have been described in cultures and tissue prints of adult human white matter (Armstrong et al., 1992). To characterize further these precursors of human oligodendrocytes, we have investigated whether they express genes playing a critical role in oligodendrocyte development. In the intact human brain, platelet-derived growth factor receptor alpha (PDGF alpha R) and myelin transcription factor 1 (MyTI) transcripts are expressed in 1-2% of cells of the oligodendrocyte lineage (OL), and clusters of such cells can be found in the periventricular region. Myelin basic protein transcripts containing exon 2 information (exon 2+ MBP), which are characteristic of the premyelinating stage, are detected in 15-20% of OL cells in vivo. When OL cells are separated from human white matter and allowed to regenerate in vitro, a much larger proportion of these cells express developmentally regulated genes, while exon 2- MBP and proteolipid protein (PLP) transcripts characteristic of mature OL cells appear transiently downregulated. Basic fibroblast growth factor (bFGF), even in the presence of PDGF, does not promote DNA synthesis in these cultured OL cells. Yet bFGF induces human oligodendrocytes to regenerate their processes rapidly in vitro and to express O4 antigens as well as exon 2+ MBP, MyTI, and PLP transcripts. While bFGF accelerates early regenerative processes, it also maintains high expression of exon 2+ MBP transcripts in OL cells for up to 2 weeks in vitro. In contrast, high levels of insulin in the absence of bFGF allow accumulation of exon 2- MBP and PLP transcripts in most OL cells at 2-3 weeks in vitro. We propose that the myelinated human brain harbors a small pool of precursors of oligodendrocytes and that growth factor-regulated phenotypic plasticity rather than mitogenic potential accounts for the regeneration of oligodendrocytes in the initial stages of demyelinating diseases such as multiple sclerosis.

Duke Scholars

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Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

August 1994

Volume

14

Issue

8

Start / End Page

4571 / 4587

Location

United States

Related Subject Headings

  • Stem Cells
  • Proteolipids
  • Oligonucleotide Probes
  • Oligodendroglia
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Myelin Basic Protein
  • Molecular Sequence Data
  • Middle Aged
  • In Situ Hybridization
 

Citation

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Gogate, N., Verma, L., Zhou, J. M., Milward, E., Rusten, R., O’Connor, M., … Dubois-Dalcq, M. (1994). Plasticity in the adult human oligodendrocyte lineage. J Neurosci, 14(8), 4571–4587. https://doi.org/10.1523/JNEUROSCI.14-08-04571.1994
Gogate, N., L. Verma, J. M. Zhou, E. Milward, R. Rusten, M. O’Connor, C. Kufta, J. Kim, L. Hudson, and M. Dubois-Dalcq. “Plasticity in the adult human oligodendrocyte lineage.J Neurosci 14, no. 8 (August 1994): 4571–87. https://doi.org/10.1523/JNEUROSCI.14-08-04571.1994.
Gogate N, Verma L, Zhou JM, Milward E, Rusten R, O’Connor M, et al. Plasticity in the adult human oligodendrocyte lineage. J Neurosci. 1994 Aug;14(8):4571–87.
Gogate, N., et al. “Plasticity in the adult human oligodendrocyte lineage.J Neurosci, vol. 14, no. 8, Aug. 1994, pp. 4571–87. Pubmed, doi:10.1523/JNEUROSCI.14-08-04571.1994.
Gogate N, Verma L, Zhou JM, Milward E, Rusten R, O’Connor M, Kufta C, Kim J, Hudson L, Dubois-Dalcq M. Plasticity in the adult human oligodendrocyte lineage. J Neurosci. 1994 Aug;14(8):4571–4587.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

August 1994

Volume

14

Issue

8

Start / End Page

4571 / 4587

Location

United States

Related Subject Headings

  • Stem Cells
  • Proteolipids
  • Oligonucleotide Probes
  • Oligodendroglia
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Myelin Basic Protein
  • Molecular Sequence Data
  • Middle Aged
  • In Situ Hybridization