Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina.
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.
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Related Subject Headings
- Mycology & Parasitology
- 3108 Plant biology
- 3107 Microbiology
- 3104 Evolutionary biology
- 0607 Plant Biology
- 0605 Microbiology
- 0603 Evolutionary Biology
Citation
Published In
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Mycology & Parasitology
- 3108 Plant biology
- 3107 Microbiology
- 3104 Evolutionary biology
- 0607 Plant Biology
- 0605 Microbiology
- 0603 Evolutionary Biology