Skip to main content
Journal cover image

Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease.

Publication ,  Journal Article
Vitek, MP; Rasool, CG; de Sauvage, F; Vitek, SM; Bartus, RT; Beer, B; Ashton, RA; Macq, AF; Maloteaux, JM; Blume, AJ
Published in: Brain Res
September 1988

Using an oligonucleotide probe, we isolated cDNA clones corresponding to the precursor of the beta-amyloid peptide (BAP) from brain libraries of 3 patients with sporadic Alzheimer's disease (AD). DNA sequencing showed that the largest cDNA clone encompasses 83% of the open reading frame proposed by Kang et al. to encode the BAP precursor (APP). cDNA clones from each of the 3 AD brain libraries were identical to the sequence of the APP-cDNAs cloned from normal adult human and fetal brain. An antisense-radiolabeled RNA copy of one of the AD clones detected a pattern of 3 gene transcripts measuring 3.5, 3.2 and 1.6 kilobases (kb) in both normal and AD brain RNAs. These data suggest that there are no mutations in or about the 42 amino acid (aa) sequence of BAP and that the accumulation of amyloid consistently found in AD may result from altered post-translational processing of APP.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

September 1988

Volume

464

Issue

2

Start / End Page

121 / 131

Location

Netherlands

Related Subject Headings

  • Reference Values
  • Oligonucleotide Probes
  • Nucleic Acid Hybridization
  • Neurology & Neurosurgery
  • Mutation
  • Molecular Sequence Data
  • Male
  • Humans
  • Hippocampus
  • DNA
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vitek, M. P., Rasool, C. G., de Sauvage, F., Vitek, S. M., Bartus, R. T., Beer, B., … Blume, A. J. (1988). Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease. Brain Res, 464(2), 121–131. https://doi.org/10.1016/0169-328x(88)90004-6
Vitek, M. P., C. G. Rasool, F. de Sauvage, S. M. Vitek, R. T. Bartus, B. Beer, R. A. Ashton, A. F. Macq, J. M. Maloteaux, and A. J. Blume. “Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease.Brain Res 464, no. 2 (September 1988): 121–31. https://doi.org/10.1016/0169-328x(88)90004-6.
Vitek MP, Rasool CG, de Sauvage F, Vitek SM, Bartus RT, Beer B, et al. Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease. Brain Res. 1988 Sep;464(2):121–31.
Vitek, M. P., et al. “Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease.Brain Res, vol. 464, no. 2, Sept. 1988, pp. 121–31. Pubmed, doi:10.1016/0169-328x(88)90004-6.
Vitek MP, Rasool CG, de Sauvage F, Vitek SM, Bartus RT, Beer B, Ashton RA, Macq AF, Maloteaux JM, Blume AJ. Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease. Brain Res. 1988 Sep;464(2):121–131.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

September 1988

Volume

464

Issue

2

Start / End Page

121 / 131

Location

Netherlands

Related Subject Headings

  • Reference Values
  • Oligonucleotide Probes
  • Nucleic Acid Hybridization
  • Neurology & Neurosurgery
  • Mutation
  • Molecular Sequence Data
  • Male
  • Humans
  • Hippocampus
  • DNA