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The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus.

Publication ,  Journal Article
Panjehpour, M; Overholt, BF; Vo-Dinh, T; Coppola, D
Published in: Lasers in surgery and medicine
July 2012

Differential Normalized Fluorescence (DNF) technique has been used to distinguish high-grade dysplasia from non-dysplastic Barrett's esophagus. This technology may assist gastroenterologists in targeting biopsies, reducing the number of biopsies using the standard protocol. In the presence of reactive atypia/inflammation, it becomes difficult for the pathologist to differentiate non-dysplastic Barrett's esophagus from Barrett's esophagus with low-grade dysplasia. Before DNF technique may be used to guide target biopsies, it is critical to know whether reactive atypia/inflammation in non-dysplastic Barrett's may result in false positives. This study was conducted to determine whether DNF technique is adversely affected by the presence of reactive atypia/inflammation in non-dysplastic Barrett's esophagus resulting in false positives.Four hundred ten-nanometer laser light was used to induce autofluorescence of Barrett's mucosa in 49 patients. The clinical study included 37 males and 12 females. This was a blinded retrospective data analysis study. A total of 303 spectra were collected and matched to non-dysplastic Barrett's biopsy results. One hundred seventy-five spectra were collected from areas with a pathology of non-dysplastic Barrett's esophagus with reactive atypia/inflammation. One hundred twenty-eight spectra were collected from areas with non-dysplastic Barrett's esophagus without reactive changes/inflammation. The spectra were analyzed using the DNF Index at 480 nm and classified as positive or negative using the threshold of -0.75 × 10(-3).Using DNF technique, 92.6% of non-dysplastic samples with reactive atypia/inflammation were classified correctly (162/175). 92.2% of non-dysplastic samples without reactive atypia/inflammation were classified correctly (118/128). Comparing the ratios of false positives among the two sample groups, there was not a statistically significant difference between the two groups.Using DNF technique for classification of non-dysplastic Barrett's mucosa does not result in false-positive readings due to reactive atypia/inflammation. Target biopsies guided by DNF technique may drastically reduce the number of pinch biopsies using the standard biopsy protocol.

Duke Scholars

Published In

Lasers in surgery and medicine

DOI

EISSN

1096-9101

ISSN

0196-8092

Publication Date

July 2012

Volume

44

Issue

5

Start / End Page

390 / 396

Related Subject Headings

  • Spectrometry, Fluorescence
  • Single-Blind Method
  • Retrospective Studies
  • Male
  • Lasers, Dye
  • Inflammation
  • Humans
  • Female
  • False Positive Reactions
  • Esophagus
 

Citation

APA
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ICMJE
MLA
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Panjehpour, M., Overholt, B. F., Vo-Dinh, T., & Coppola, D. (2012). The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus. Lasers in Surgery and Medicine, 44(5), 390–396. https://doi.org/10.1002/lsm.22033
Panjehpour, Masoud, Bergein F. Overholt, Tuan Vo-Dinh, and Domenico Coppola. “The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus.Lasers in Surgery and Medicine 44, no. 5 (July 2012): 390–96. https://doi.org/10.1002/lsm.22033.
Panjehpour M, Overholt BF, Vo-Dinh T, Coppola D. The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus. Lasers in surgery and medicine. 2012 Jul;44(5):390–6.
Panjehpour, Masoud, et al. “The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus.Lasers in Surgery and Medicine, vol. 44, no. 5, July 2012, pp. 390–96. Epmc, doi:10.1002/lsm.22033.
Panjehpour M, Overholt BF, Vo-Dinh T, Coppola D. The effect of reactive atypia/inflammation on the laser-induced fluorescence diagnosis of non-dysplastic Barrett's esophagus. Lasers in surgery and medicine. 2012 Jul;44(5):390–396.
Journal cover image

Published In

Lasers in surgery and medicine

DOI

EISSN

1096-9101

ISSN

0196-8092

Publication Date

July 2012

Volume

44

Issue

5

Start / End Page

390 / 396

Related Subject Headings

  • Spectrometry, Fluorescence
  • Single-Blind Method
  • Retrospective Studies
  • Male
  • Lasers, Dye
  • Inflammation
  • Humans
  • Female
  • False Positive Reactions
  • Esophagus