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Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing

Publication ,  Journal Article
Gregas, MK; Yan, F; Scaffidi, J; Wang, HN; Khoury, C; Zhang, Y; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
December 1, 2007

A critical aspect of the use of nanoprobes for intracellular studies in chemical and biological sensing involves a fundamental understanding of their uptake and trajectory in cells. In this study, we describe experiments using surfaceenhanced Raman scattering (SERS) spectroscopy and mapping to track cellular uptake of plasmonics-active labeled nanoparticles. Three different Raman-active labels with positive, negative, and neutral charges were conjugated to silver colloidal nanoparticles with the aim of spatially and temporally profiling intracellular delivery and tracking of nanoprobes during uptake in single mammalian cells. 1-D Raman spectra and 2-D Raman mapping are used to identify and locate the probes via their SERS signal intensities. Because Raman spectroscopy is very specific for identification of chemical and molecular signatures, the development of functionalized plasmonics-active nanoprobes capable of exploring intracellular spaces and processes has the ability to provide specific information on the effects of biological and chemical pollutants in the intracellular environment. The results indicate that this technique will allow study of when, where, and how these substances affect cells and living organisms.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

December 1, 2007

Volume

6755

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Gregas, M. K., Yan, F., Scaffidi, J., Wang, H. N., Khoury, C., Zhang, Y., & Vo-Dinh, T. (2007). Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing. Proceedings of SPIE - The International Society for Optical Engineering, 6755. https://doi.org/10.1117/12.758287
Gregas, M. K., F. Yan, J. Scaffidi, H. N. Wang, C. Khoury, Y. Zhang, and T. Vo-Dinh. “Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing.” Proceedings of SPIE - The International Society for Optical Engineering 6755 (December 1, 2007). https://doi.org/10.1117/12.758287.
Gregas MK, Yan F, Scaffidi J, Wang HN, Khoury C, Zhang Y, et al. Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing. Proceedings of SPIE - The International Society for Optical Engineering. 2007 Dec 1;6755.
Gregas, M. K., et al. “Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 6755, Dec. 2007. Scopus, doi:10.1117/12.758287.
Gregas MK, Yan F, Scaffidi J, Wang HN, Khoury C, Zhang Y, Vo-Dinh T. Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing. Proceedings of SPIE - The International Society for Optical Engineering. 2007 Dec 1;6755.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

December 1, 2007

Volume

6755

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering