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Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice.

Publication ,  Journal Article
Hasegawa, H; Wang, F
Published in: J Comp Neurol
December 1, 2008

Somatosensory neurons are classified into three main types according to their modalities: nociceptive, thermal, and mechanosensory. Within each modality group, neurons can be further divided into morphologically and functionally distinct subclasses. Here we show that heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2 (HS3ST-2) is a marker for specific subsets of TrkC-expressing cutaneous low-threshold mechanosensory and proprioceptive mechanosensory neurons. Two-color in situ analysis revealed that almost all HS3ST-2 signals colocalized with TrkC but not with TrkA or TrkB mRNA. To visualize the morphological subtypes of HS3ST-2/TrkC-expressing neurons, we generated a HS3ST-2-hPLAP knock-in mouse line, in which HS3ST-2-expressing neurons and their projections are labeled by human placental alkaline phosphatase (hPLAP). AP staining in these mice demonstrated that sensory endings of muscle spindles and Golgi tendon organs as well as the cutaneous mechanosensory Merkel and longitudinal lanceolate endings in the whiskers are strongly positive for hPLAP activity. In contrast, no nociceptive endings are labeled. In the glabrous and hairy skin, rare Merkel endings and transverse lanceolate endings are weakly stained. During development, each type of nerve endings forms at different time point. Muscle innervations differentiate first, followed by formation of cutaneous sensory endings. Our results revealed the subtype identities of TrkC-positive mechanosensory neurons and demonstrated the usefulness of HS3ST-2 as a genetic marker for these subclasses of neurons.

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Published In

J Comp Neurol

DOI

EISSN

1096-9861

Publication Date

December 1, 2008

Volume

511

Issue

4

Start / End Page

543 / 556

Location

United States

Related Subject Headings

  • Vibrissae
  • Trigeminal Ganglion
  • Sulfotransferases
  • Sensory Receptor Cells
  • Receptor, trkC
  • RNA, Messenger
  • Proteins
  • Polymerase Chain Reaction
  • Neurology & Neurosurgery
  • Muscle, Skeletal
 

Citation

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Hasegawa, H., & Wang, F. (2008). Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice. J Comp Neurol, 511(4), 543–556. https://doi.org/10.1002/cne.21862
Hasegawa, Hiroshi, and Fan Wang. “Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice.J Comp Neurol 511, no. 4 (December 1, 2008): 543–56. https://doi.org/10.1002/cne.21862.
Hasegawa H, Wang F. Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice. J Comp Neurol. 2008 Dec 1;511(4):543–56.
Hasegawa, Hiroshi, and Fan Wang. “Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice.J Comp Neurol, vol. 511, no. 4, Dec. 2008, pp. 543–56. Pubmed, doi:10.1002/cne.21862.
Hasegawa H, Wang F. Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice. J Comp Neurol. 2008 Dec 1;511(4):543–556.
Journal cover image

Published In

J Comp Neurol

DOI

EISSN

1096-9861

Publication Date

December 1, 2008

Volume

511

Issue

4

Start / End Page

543 / 556

Location

United States

Related Subject Headings

  • Vibrissae
  • Trigeminal Ganglion
  • Sulfotransferases
  • Sensory Receptor Cells
  • Receptor, trkC
  • RNA, Messenger
  • Proteins
  • Polymerase Chain Reaction
  • Neurology & Neurosurgery
  • Muscle, Skeletal