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Transplantation of full-thickness retina in the rhodopsin transgenic pig.

Publication ,  Journal Article
Ghosh, F; Wong, F; Johansson, K; Bruun, A; Petters, RM
Published in: Retina
February 2004

PURPOSE: To establish the morphology of full-thickness neuroretinal grafts transplanted to hosts with degenerative photoreceptor disease. METHODS: Twenty rhodopsin transgenic pigs received a neuroretinal sheet from a neonatal normal pig in one eye. Following vitrectomy and retinotomy with bleb formation, the grafts were positioned inside the bleb between the host neuroretina and retinal pigment epithelium. After a survival time of 4 months, eye specimens were studied by light and electron microscopy as well as with immunohistochemical markers. RESULTS: One eye developed endophthalmitis in the immediate postoperative period and was terminated. Laminated grafts with correct polarity were found in 13 of the remaining 19 eyes. In most cases, these grafts had well-developed organized photoreceptors with outer segments apposed to the host retinal pigment epithelium. The inner layers of the graft contained mostly Müller cells. Both eyes of the hosts had a reduction of photoreceptor cells in most of the retina, while inner layers remained relatively intact. CONCLUSIONS: Full-thickness neuroretinal grafts can be transplanted to a large animal host with photoreceptor degeneration. The transplantation procedure is relatively atraumatic to both graft and host tissue, and the grafts survive well for at least 4 months. The graft and host retina does not seem to form extensive neuronal contacts, and future work must be directed at stimulating such activity without disrupting the retinal neuronal organization.

Duke Scholars

Published In

Retina

DOI

ISSN

0275-004X

Publication Date

February 2004

Volume

24

Issue

1

Start / End Page

98 / 109

Location

United States

Related Subject Headings

  • Vimentin
  • Swine
  • Rhodopsin
  • Retinal Degeneration
  • Retina
  • Recoverin
  • Protein Kinase C
  • Pigment Epithelium of Eye
  • Photoreceptor Cells, Vertebrate
  • Ophthalmology & Optometry
 

Citation

APA
Chicago
ICMJE
MLA
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Ghosh, F., Wong, F., Johansson, K., Bruun, A., & Petters, R. M. (2004). Transplantation of full-thickness retina in the rhodopsin transgenic pig. Retina, 24(1), 98–109. https://doi.org/10.1097/00006982-200402000-00014
Ghosh, Fredrik, Fulton Wong, Kjell Johansson, Anitha Bruun, and Robert M. Petters. “Transplantation of full-thickness retina in the rhodopsin transgenic pig.Retina 24, no. 1 (February 2004): 98–109. https://doi.org/10.1097/00006982-200402000-00014.
Ghosh F, Wong F, Johansson K, Bruun A, Petters RM. Transplantation of full-thickness retina in the rhodopsin transgenic pig. Retina. 2004 Feb;24(1):98–109.
Ghosh, Fredrik, et al. “Transplantation of full-thickness retina in the rhodopsin transgenic pig.Retina, vol. 24, no. 1, Feb. 2004, pp. 98–109. Pubmed, doi:10.1097/00006982-200402000-00014.
Ghosh F, Wong F, Johansson K, Bruun A, Petters RM. Transplantation of full-thickness retina in the rhodopsin transgenic pig. Retina. 2004 Feb;24(1):98–109.

Published In

Retina

DOI

ISSN

0275-004X

Publication Date

February 2004

Volume

24

Issue

1

Start / End Page

98 / 109

Location

United States

Related Subject Headings

  • Vimentin
  • Swine
  • Rhodopsin
  • Retinal Degeneration
  • Retina
  • Recoverin
  • Protein Kinase C
  • Pigment Epithelium of Eye
  • Photoreceptor Cells, Vertebrate
  • Ophthalmology & Optometry