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Dual action of TGF-beta1 on nasal-polyp derived fibroblasts.

Publication ,  Journal Article
Little, SC; Early, SB; Woodard, CR; Shonka, DC; Han, JK; Borish, L; Steinke, JW
Published in: Laryngoscope
February 2008

OBJECTIVES: Transforming growth factor beta-1 (TGF-beta1) is a known fibrogenic factor with immunosuppressive properties. We wanted to determine the effect of stimulation with TGF-beta1 on nasal polyp-derived fibroblasts and assess the role this molecule would have in polyp formation and growth. STUDY DESIGN: Nasal-polyp derived fibroblasts were cultured with or without TGF-beta1, and proliferation and cytokine secretion were measured. METHODS: Fibroblasts were isolated from nasal polyps following endoscopic surgery. Cells were plated and grown until confluent, after which they were split and used in assays. Cells were stimulated with TGF- beta1 and mRNA collected after 16 hours, supernatants after 72 hours, and proliferation measured after 96 hours of culture. RESULTS: TGF-beta1 significantly (P < .02) increased proliferation of nasal-polyp derived fibroblasts. We examined the expression of inflammatory cytokines and found that TGF-beta1 decreased expression of CCL2 (MCP-1), CCL5 (RANTES), CCL11 (eotaxin), granulocyte-colony stimulating factor (G-CSF), and GM-CSF (P < .05). In contrast, incubation with TGF-beta1 increased fibronectin, procollagen, vascular endothelial growth factor (VEGF), and TGF-beta2 protein production (P < .05). For select samples, we confirmed that the increased protein production was due to increased mRNA expression. CONCLUSION: These studies suggest that TGF-beta1 expression in polyp tissue can have dual effects. One role is to act as an anti-inflammatory agent shown by the ability to inhibit pro-inflammatory mRNA and protein production. At the same time, TGF-beta1 expression leads to increases in factors involved in fibrosis and angiogenesis, promoting remodeling and cell growth.

Duke Scholars

Published In

Laryngoscope

DOI

ISSN

0023-852X

Publication Date

February 2008

Volume

118

Issue

2

Start / End Page

320 / 324

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta1
  • Time Factors
  • Signal Transduction
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Otorhinolaryngology
  • Nasal Polyps
  • Humans
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Fibronectins
 

Citation

APA
Chicago
ICMJE
MLA
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Little, S. C., Early, S. B., Woodard, C. R., Shonka, D. C., Han, J. K., Borish, L., & Steinke, J. W. (2008). Dual action of TGF-beta1 on nasal-polyp derived fibroblasts. Laryngoscope, 118(2), 320–324. https://doi.org/10.1097/MLG.0b013e318159cc0b
Little, Stewart C., S Brandon Early, Charles R. Woodard, David C. Shonka, Joseph K. Han, Larry Borish, and John W. Steinke. “Dual action of TGF-beta1 on nasal-polyp derived fibroblasts.Laryngoscope 118, no. 2 (February 2008): 320–24. https://doi.org/10.1097/MLG.0b013e318159cc0b.
Little SC, Early SB, Woodard CR, Shonka DC, Han JK, Borish L, et al. Dual action of TGF-beta1 on nasal-polyp derived fibroblasts. Laryngoscope. 2008 Feb;118(2):320–4.
Little, Stewart C., et al. “Dual action of TGF-beta1 on nasal-polyp derived fibroblasts.Laryngoscope, vol. 118, no. 2, Feb. 2008, pp. 320–24. Pubmed, doi:10.1097/MLG.0b013e318159cc0b.
Little SC, Early SB, Woodard CR, Shonka DC, Han JK, Borish L, Steinke JW. Dual action of TGF-beta1 on nasal-polyp derived fibroblasts. Laryngoscope. 2008 Feb;118(2):320–324.
Journal cover image

Published In

Laryngoscope

DOI

ISSN

0023-852X

Publication Date

February 2008

Volume

118

Issue

2

Start / End Page

320 / 324

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta1
  • Time Factors
  • Signal Transduction
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Otorhinolaryngology
  • Nasal Polyps
  • Humans
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Fibronectins