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Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses.

Publication ,  Journal Article
Engelhardt, JF; Yang, Y; Stratford-Perricaudet, LD; Allen, ED; Kozarsky, K; Perricaudet, M; Yankaskas, JR; Wilson, JM
Published in: Nature genetics
May 1993

We describe the use of a human bronchial xenograft model for studying the efficiency and biology of in vivo gene transfer into human bronchial epithelia with recombinant E1 deleted adenoviruses. All cell types in the surface epithelium except basal cells efficiently expressed the adenoviral transduced recombinant genes, lacZ and CFTR, for 3-5 weeks. Stable transgene expression was associated with high level expression of the early adenoviral gene, E2a, in a subset of transgene expressing cells and virtually undetectable expression of the late adenoviral genes encoding the structural proteins, hexon and fiber. These studies begin to address important issues that relate to safety and in vivo efficacy of recombinant adenoviruses for gene delivery into the human airway.

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Published In

Nature genetics

DOI

EISSN

1546-1718

ISSN

1061-4036

Publication Date

May 1993

Volume

4

Issue

1

Start / End Page

27 / 34

Related Subject Headings

  • Viral Proteins
  • Transplantation, Heterotopic
  • Transplantation, Heterologous
  • Transfection
  • Trachea
  • Safety
  • Recombinant Fusion Proteins
  • Rats, Inbred F344
  • Rats
  • Mice, Nude
 

Citation

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Engelhardt, J. F., Yang, Y., Stratford-Perricaudet, L. D., Allen, E. D., Kozarsky, K., Perricaudet, M., … Wilson, J. M. (1993). Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses. Nature Genetics, 4(1), 27–34. https://doi.org/10.1038/ng0593-27
Engelhardt, J. F., Y. Yang, L. D. Stratford-Perricaudet, E. D. Allen, K. Kozarsky, M. Perricaudet, J. R. Yankaskas, and J. M. Wilson. “Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses.Nature Genetics 4, no. 1 (May 1993): 27–34. https://doi.org/10.1038/ng0593-27.
Engelhardt JF, Yang Y, Stratford-Perricaudet LD, Allen ED, Kozarsky K, Perricaudet M, et al. Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses. Nature genetics. 1993 May;4(1):27–34.
Engelhardt, J. F., et al. “Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses.Nature Genetics, vol. 4, no. 1, May 1993, pp. 27–34. Epmc, doi:10.1038/ng0593-27.
Engelhardt JF, Yang Y, Stratford-Perricaudet LD, Allen ED, Kozarsky K, Perricaudet M, Yankaskas JR, Wilson JM. Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses. Nature genetics. 1993 May;4(1):27–34.

Published In

Nature genetics

DOI

EISSN

1546-1718

ISSN

1061-4036

Publication Date

May 1993

Volume

4

Issue

1

Start / End Page

27 / 34

Related Subject Headings

  • Viral Proteins
  • Transplantation, Heterotopic
  • Transplantation, Heterologous
  • Transfection
  • Trachea
  • Safety
  • Recombinant Fusion Proteins
  • Rats, Inbred F344
  • Rats
  • Mice, Nude