High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.
A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.
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- Stents
- Steel
- Rats, Sprague-Dawley
- Rats
- Polymers
- Nanotechnology
- Models, Biological
- Metal Nanoparticles
- Male
- Magnetics
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Stents
- Steel
- Rats, Sprague-Dawley
- Rats
- Polymers
- Nanotechnology
- Models, Biological
- Metal Nanoparticles
- Male
- Magnetics