Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells.
Publication
, Journal Article
Otto, JC; York, JD
Published in: Methods Mol Biol
2010
Lipid-derived inositol phosphates (InsPs) comprise a family of second messengers that arise through the action of six classes of InsP kinases, generally referred to as IPKs. Genetic studies have indicated that InsPs play critical roles in embryonic development, but the mechanisms of action for InsPs in mammalian cellular function are largely unknown. This chapter outlines a method for manipulating cellular InsP profiles through the coexpression of a constitutively active G protein and various IPKs. It provides a mechanism by which the metabolism of a variety of InsPs can be upregulated, enabling the evaluation of the effects of these InsPs on cellular functions.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2010
Volume
645
Start / End Page
47 / 60
Location
United States
Related Subject Headings
- Type C Phospholipases
- Mutation
- Isotope Labeling
- Inositol Phosphates
- Humans
- GTP-Binding Proteins
- Diphosphates
- Developmental Biology
- Chromatography, High Pressure Liquid
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Otto, J. C., & York, J. D. (2010). Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells. Methods Mol Biol, 645, 47–60. https://doi.org/10.1007/978-1-60327-175-2_3
Otto, James C., and John D. York. “Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells.” Methods Mol Biol 645 (2010): 47–60. https://doi.org/10.1007/978-1-60327-175-2_3.
Otto JC, York JD. Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells. Methods Mol Biol. 2010;645:47–60.
Otto, James C., and John D. York. “Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells.” Methods Mol Biol, vol. 645, 2010, pp. 47–60. Pubmed, doi:10.1007/978-1-60327-175-2_3.
Otto JC, York JD. Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells. Methods Mol Biol. 2010;645:47–60.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2010
Volume
645
Start / End Page
47 / 60
Location
United States
Related Subject Headings
- Type C Phospholipases
- Mutation
- Isotope Labeling
- Inositol Phosphates
- Humans
- GTP-Binding Proteins
- Diphosphates
- Developmental Biology
- Chromatography, High Pressure Liquid
- Cell Line