Skip to main content
Journal cover image

Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates.

Publication ,  Journal Article
Lee, Y-S; Mulugu, S; York, JD; O'Shea, EK
Published in: Science
April 6, 2007

In budding yeast, phosphate starvation triggers inhibition of the Pho80-Pho85 cyclin-cyclin-dependent kinase (CDK) complex by the CDK inhibitor Pho81, leading to expression of genes involved in nutrient homeostasis. We isolated myo-d-inositol heptakisphosphate (IP7) as a cellular component that stimulates Pho81-dependent inhibition of Pho80-Pho85. IP7 is necessary for Pho81-dependent inhibition of Pho80-Pho85 in vitro. Moreover, intracellular concentrations of IP7 increased upon phosphate starvation, and yeast mutants defective in IP7 production failed to inhibit Pho80-Pho85 in response to phosphate starvation. These observations reveal regulation of a cyclin-CDK complex by a metabolite and suggest that a complex metabolic network mediates signaling of phosphate availability.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 6, 2007

Volume

316

Issue

5821

Start / End Page

109 / 112

Location

United States

Related Subject Headings

  • Transcription Factors
  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repressor Proteins
  • Recombinant Proteins
  • Phosphotransferases (Phosphate Group Acceptor)
  • Inositol Phosphates
  • General Science & Technology
  • DNA-Binding Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lee, Y.-S., Mulugu, S., York, J. D., & O’Shea, E. K. (2007). Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science, 316(5821), 109–112. https://doi.org/10.1126/science.1139080
Lee, Young-Sam, Sashidhar Mulugu, John D. York, and Erin K. O’Shea. “Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates.Science 316, no. 5821 (April 6, 2007): 109–12. https://doi.org/10.1126/science.1139080.
Lee Y-S, Mulugu S, York JD, O’Shea EK. Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science. 2007 Apr 6;316(5821):109–12.
Lee, Young-Sam, et al. “Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates.Science, vol. 316, no. 5821, Apr. 2007, pp. 109–12. Pubmed, doi:10.1126/science.1139080.
Lee Y-S, Mulugu S, York JD, O’Shea EK. Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science. 2007 Apr 6;316(5821):109–112.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 6, 2007

Volume

316

Issue

5821

Start / End Page

109 / 112

Location

United States

Related Subject Headings

  • Transcription Factors
  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repressor Proteins
  • Recombinant Proteins
  • Phosphotransferases (Phosphate Group Acceptor)
  • Inositol Phosphates
  • General Science & Technology
  • DNA-Binding Proteins