Skip to main content

Nitric oxide modulates branching morphogenesis in fetal rat lung explants.

Publication ,  Journal Article
Young, SL; Evans, K; Eu, JP
Published in: Am J Physiol Lung Cell Mol Physiol
March 2002

Cells of the developing lung express the constitutive nitric oxide synthases (NOSs) I and III. The developmental importance of these enzymes is largely unknown, although a role for nitric oxide (NO) in the regulation of pulmonary blood flow at birth is established. Known effects of NO on transcription factors, apoptosis, and cellular proliferation, plus the time and spatial limits of pulmonary NOS expression, suggest that NO might influence lung development. We tested the potential of NO to modulate lung branching morphogenesis by exposing lung explants from gestational day 13 rat fetuses to varying doses of several NO donors (NONO-ate). We counted the number of airway branches that were added between the first and 72nd h of culture. NO released only from a NONO-ate with a long half-life [(Z)-1-[2-(2-aminoethyl)-N-(2-ammonioethyl)amino]-diazen-1-ium-1,2-diolate-NO], increased branching in ambient O(2) by twofold. The NO effect was not mimicked with a cyclic guanine monophosphate analog; nonspecific NOS inhibitors in millimolar concentrations inhibited branching. We conclude that endogenous and exogenous NO can modulate branching morphogenesis in the rat lung.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

March 2002

Volume

282

Issue

3

Start / End Page

L379 / L385

Location

United States

Related Subject Headings

  • omega-N-Methylarginine
  • Triazenes
  • Time Factors
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Organ Culture Techniques
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Nitric Oxide Donors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Young, S. L., Evans, K., & Eu, J. P. (2002). Nitric oxide modulates branching morphogenesis in fetal rat lung explants. Am J Physiol Lung Cell Mol Physiol, 282(3), L379–L385. https://doi.org/10.1152/ajplung.00462.2000
Young, Stephen L., Katherine Evans, and Jerry P. Eu. “Nitric oxide modulates branching morphogenesis in fetal rat lung explants.Am J Physiol Lung Cell Mol Physiol 282, no. 3 (March 2002): L379–85. https://doi.org/10.1152/ajplung.00462.2000.
Young SL, Evans K, Eu JP. Nitric oxide modulates branching morphogenesis in fetal rat lung explants. Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L379–85.
Young, Stephen L., et al. “Nitric oxide modulates branching morphogenesis in fetal rat lung explants.Am J Physiol Lung Cell Mol Physiol, vol. 282, no. 3, Mar. 2002, pp. L379–85. Pubmed, doi:10.1152/ajplung.00462.2000.
Young SL, Evans K, Eu JP. Nitric oxide modulates branching morphogenesis in fetal rat lung explants. Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L379–L385.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

March 2002

Volume

282

Issue

3

Start / End Page

L379 / L385

Location

United States

Related Subject Headings

  • omega-N-Methylarginine
  • Triazenes
  • Time Factors
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • Organ Culture Techniques
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Nitric Oxide Donors