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Three-dimensional reconstruction of tubular myelin.

Publication ,  Journal Article
Young, SL; Fram, EK; Larson, EW
Published in: Exp Lung Res
1992

The lipid and protein components of pulmonary surfactant are synthesized by alveolar type II cells and stored in their secretory granules, the lamellar bodies. Tubular myelin is a highly ordered surfactant structure that is lung specific and produced in the alveolar airspace. Recent work identified Ca2+, surfactant apoproteins A and B, and saturated phospholipids as necessary for tubular myelin structure and function. We have used serial ultrathin sections from fetal rat lungs and reconstructed the three-dimensional structure of tubular myelin. The spheres of tubular myelin we viewed were 2-3 microns in diameter and were surrounded by up to 20 lamellar bodies, each apparently simultaneously contributing material to the tubules. We measured a long-range symmetry of the tubules, which were folded about a central axis and showed a repeated crossing of individual tubes from one side to the other of the large structure. The tubes appeared closed on their outer edges and there was a delay in the penetration of tubules by cationic ferritin added to tissue slices containing the tubular myelin, although within a few minutes tracer appeared in the lumina and on the walls of the tubules. The three-dimensional images were compatible with existing views of tubular myelin.

Duke Scholars

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Published In

Exp Lung Res

DOI

ISSN

0190-2148

Publication Date

1992

Volume

18

Issue

4

Start / End Page

497 / 504

Location

England

Related Subject Headings

  • Respiratory System
  • Rats, Inbred Strains
  • Rats
  • Pulmonary Surfactants
  • Proteins
  • Myelin Sheath
  • Image Processing, Computer-Assisted
  • Female
  • Biological Products
  • Animals
 

Citation

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Young, S. L., Fram, E. K., & Larson, E. W. (1992). Three-dimensional reconstruction of tubular myelin. Exp Lung Res, 18(4), 497–504. https://doi.org/10.3109/01902149209064342
Young, S. L., E. K. Fram, and E. W. Larson. “Three-dimensional reconstruction of tubular myelin.Exp Lung Res 18, no. 4 (1992): 497–504. https://doi.org/10.3109/01902149209064342.
Young SL, Fram EK, Larson EW. Three-dimensional reconstruction of tubular myelin. Exp Lung Res. 1992;18(4):497–504.
Young, S. L., et al. “Three-dimensional reconstruction of tubular myelin.Exp Lung Res, vol. 18, no. 4, 1992, pp. 497–504. Pubmed, doi:10.3109/01902149209064342.
Young SL, Fram EK, Larson EW. Three-dimensional reconstruction of tubular myelin. Exp Lung Res. 1992;18(4):497–504.
Journal cover image

Published In

Exp Lung Res

DOI

ISSN

0190-2148

Publication Date

1992

Volume

18

Issue

4

Start / End Page

497 / 504

Location

England

Related Subject Headings

  • Respiratory System
  • Rats, Inbred Strains
  • Rats
  • Pulmonary Surfactants
  • Proteins
  • Myelin Sheath
  • Image Processing, Computer-Assisted
  • Female
  • Biological Products
  • Animals