Skip to main content

Rat lung surfactant kinetics biochemical and morphometric correlation.

Publication ,  Journal Article
Young, SL; Kremers, SA; Apple, JS; Crapo, JD; Brumley, GW
Published in: J Appl Physiol Respir Environ Exerc Physiol
August 1981

Biochemical and morphometric techniques were combined to estimate the distribution and flux or rat lung disaturated phosphatidylcholine (DSPC) in surfactant pathways. We found 126 +/- 8 X 10(6) alveolar epithelial type II (AEP II) cells per lung, each containing 150 +/- 30 lamellar bodies (LB). High yields of LB were isolated by centrifugation of lung homogenates with a discontinuous sucrose density gradient. Measurement of DSPC in LB and alveolar lavage (LV) material demonstrated that only 30% (LB 14%, LV 16%) of lung DSPC was present in these surfactant compartments. Radiolabeling of lung DSPC with [14C]palmitate demonstrated a product-precursor-relationship between LB and LV and a surfactant-associated DSPC flux of 90 microgram-lung-1 X h-1. We conclude that, although only 16% of lung DSPC is in an intracellular surfactant pathway at any time, all this DSPC is ultimately secreted onto the alveolar surface.

Duke Scholars

Published In

J Appl Physiol Respir Environ Exerc Physiol

DOI

ISSN

0161-7567

Publication Date

August 1981

Volume

51

Issue

2

Start / End Page

248 / 253

Location

United States

Related Subject Headings

  • Rats
  • Pulmonary Surfactants
  • Pulmonary Alveoli
  • Physiology
  • Male
  • Cytoplasmic Granules
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Young, S. L., Kremers, S. A., Apple, J. S., Crapo, J. D., & Brumley, G. W. (1981). Rat lung surfactant kinetics biochemical and morphometric correlation. J Appl Physiol Respir Environ Exerc Physiol, 51(2), 248–253. https://doi.org/10.1152/jappl.1981.51.2.248
Young, S. L., S. A. Kremers, J. S. Apple, J. D. Crapo, and G. W. Brumley. “Rat lung surfactant kinetics biochemical and morphometric correlation.J Appl Physiol Respir Environ Exerc Physiol 51, no. 2 (August 1981): 248–53. https://doi.org/10.1152/jappl.1981.51.2.248.
Young SL, Kremers SA, Apple JS, Crapo JD, Brumley GW. Rat lung surfactant kinetics biochemical and morphometric correlation. J Appl Physiol Respir Environ Exerc Physiol. 1981 Aug;51(2):248–53.
Young, S. L., et al. “Rat lung surfactant kinetics biochemical and morphometric correlation.J Appl Physiol Respir Environ Exerc Physiol, vol. 51, no. 2, Aug. 1981, pp. 248–53. Pubmed, doi:10.1152/jappl.1981.51.2.248.
Young SL, Kremers SA, Apple JS, Crapo JD, Brumley GW. Rat lung surfactant kinetics biochemical and morphometric correlation. J Appl Physiol Respir Environ Exerc Physiol. 1981 Aug;51(2):248–253.

Published In

J Appl Physiol Respir Environ Exerc Physiol

DOI

ISSN

0161-7567

Publication Date

August 1981

Volume

51

Issue

2

Start / End Page

248 / 253

Location

United States

Related Subject Headings

  • Rats
  • Pulmonary Surfactants
  • Pulmonary Alveoli
  • Physiology
  • Male
  • Cytoplasmic Granules
  • Animals
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences