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Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1.

Publication ,  Journal Article
Shin, J; Zhang, P; Wang, S; Wu, J; Guan, Z; Zhong, X-P
Published in: Eur J Immunol
January 2013

Mast cells play a critical role in the pathogenesis of allergic diseases; however, how mast cell function is regulated is still not well understood. Both phosphatidic acid (PA) and diacylglycerol (DAG) are important secondary messengers involved in mast cell activ-ation. Lipin1 is a phosphatidate phosphatase that hydrolyzes PA to produce DAG, but the role of lipin1 in mast cell function has been thus far unknown. Here we show that lipin1 is an important and selective inhibitor of mast cell degranulation. Lipin1 deficiency enhanced FcεRI-mediated β-hexosaminidase and prostaglandin D2 release from mast cells in vitro and exacerbated the passive systemic anaphylaxis reaction in vivo. Lipin1 deficiency, however, did not exert obvious effects on IL-6 or TNF-α production following FcεRI engagement. FcεRI-induced PKC and SNAP-23 phosphorylation were augmented in the lipin1-deficient mast cells. Moreover, inhibition of PKC activity reduced SNAP-23 phosphorylation and mast cell degranulation in lipin1-deficient mast cells. Together, our findings suggest that lipin1 may negatively control mast cell degranulation and the anaphylactic response through inhibiting the PKC-SNAP-23 pathway.

Duke Scholars

Published In

Eur J Immunol

DOI

EISSN

1521-4141

Publication Date

January 2013

Volume

43

Issue

1

Start / End Page

240 / 248

Location

Germany

Related Subject Headings

  • beta-N-Acetylhexosaminidases
  • Signal Transduction
  • Receptors, IgE
  • Qc-SNARE Proteins
  • Qb-SNARE Proteins
  • Protein Kinase C
  • Prostaglandin D2
  • Phosphorylation
  • Phosphatidate Phosphatase
  • Nuclear Proteins
 

Citation

APA
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ICMJE
MLA
NLM
Shin, J., Zhang, P., Wang, S., Wu, J., Guan, Z., & Zhong, X.-P. (2013). Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1. Eur J Immunol, 43(1), 240–248. https://doi.org/10.1002/eji.201242571
Shin, Jinwook, Ping Zhang, Shang Wang, Jinhong Wu, Ziqiang Guan, and Xiao-Ping Zhong. “Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1.Eur J Immunol 43, no. 1 (January 2013): 240–48. https://doi.org/10.1002/eji.201242571.
Shin J, Zhang P, Wang S, Wu J, Guan Z, Zhong X-P. Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1. Eur J Immunol. 2013 Jan;43(1):240–8.
Shin, Jinwook, et al. “Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1.Eur J Immunol, vol. 43, no. 1, Jan. 2013, pp. 240–48. Pubmed, doi:10.1002/eji.201242571.
Shin J, Zhang P, Wang S, Wu J, Guan Z, Zhong X-P. Negative control of mast cell degranulation and the anaphylactic response by the phosphatase lipin1. Eur J Immunol. 2013 Jan;43(1):240–248.
Journal cover image

Published In

Eur J Immunol

DOI

EISSN

1521-4141

Publication Date

January 2013

Volume

43

Issue

1

Start / End Page

240 / 248

Location

Germany

Related Subject Headings

  • beta-N-Acetylhexosaminidases
  • Signal Transduction
  • Receptors, IgE
  • Qc-SNARE Proteins
  • Qb-SNARE Proteins
  • Protein Kinase C
  • Prostaglandin D2
  • Phosphorylation
  • Phosphatidate Phosphatase
  • Nuclear Proteins