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Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression

Publication ,  Journal Article
Li, Y; Kulis, M; Pons, L; Zhong, XP; Burks, AW
Published in: Food and Agricultural Immunology
December 1, 2011

Background. Peanut allergy is driven by peanut-specific Th2 cells. Ara h 2, a peanut 2S albumin, is one of the major peanut allergens. Methods. An Ara h 2-specific T cell line was generated from peanut sensitised C3H/HeJ mice and used to analyse allergen-induced cytokine secretion and activation of signalling molecules. Results. Peanut stimulated splenocytes from peanut sensitised mice had strong proliferation responses and highly elevated IL-4 secretion. The Ara h 2-specific T cells generated from sensitised mice were mainly CD4+ T helper cells with a Th2 cytokine profile. The expression of CD154 on the surface of these T cell clones was detected following Ara h 2 stimulation. Ara h 2 stimulation induced phosphorylation of ZAP-70 and Erk1/2 in the T cells. Conclusions. CD154 is a useful marker for identifying activated, allergen-specific T cells. The Ras-Erk mitogen-activated protein kinase pathway is involved in the activation of Ara h 2-specific Th2 cells. © 2011 Copyright Taylor and Francis Group, LLC.

Duke Scholars

Published In

Food and Agricultural Immunology

DOI

EISSN

1465-3443

ISSN

0954-0105

Publication Date

December 1, 2011

Volume

22

Issue

4

Start / End Page

335 / 344

Related Subject Headings

  • Analytical Chemistry
  • 3204 Immunology
  • 3006 Food sciences
  • 1107 Immunology
  • 0908 Food Sciences
 

Citation

APA
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ICMJE
MLA
NLM
Li, Y., Kulis, M., Pons, L., Zhong, X. P., & Burks, A. W. (2011). Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression. Food and Agricultural Immunology, 22(4), 335–344. https://doi.org/10.1080/09540105.2011.579591
Li, Y., M. Kulis, L. Pons, X. P. Zhong, and A. W. Burks. “Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression.” Food and Agricultural Immunology 22, no. 4 (December 1, 2011): 335–44. https://doi.org/10.1080/09540105.2011.579591.
Li Y, Kulis M, Pons L, Zhong XP, Burks AW. Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression. Food and Agricultural Immunology. 2011 Dec 1;22(4):335–44.
Li, Y., et al. “Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression.” Food and Agricultural Immunology, vol. 22, no. 4, Dec. 2011, pp. 335–44. Scopus, doi:10.1080/09540105.2011.579591.
Li Y, Kulis M, Pons L, Zhong XP, Burks AW. Peanut allergen Ara h 2-specific T cells are activated via Ras-Erk MAP kinase pathway signalling and identified by CD154 expression. Food and Agricultural Immunology. 2011 Dec 1;22(4):335–344.

Published In

Food and Agricultural Immunology

DOI

EISSN

1465-3443

ISSN

0954-0105

Publication Date

December 1, 2011

Volume

22

Issue

4

Start / End Page

335 / 344

Related Subject Headings

  • Analytical Chemistry
  • 3204 Immunology
  • 3006 Food sciences
  • 1107 Immunology
  • 0908 Food Sciences