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Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production.

Publication ,  Journal Article
Spasojevic, I; Liochev, SI; Fridovich, I
Published in: Arch Biochem Biophys
January 15, 2000

The use of lucigenin luminescence as a measure of ¿O-(2) has been questioned because lucigenin has been shown to be capable of mediating the production of O-(2). This being the case, lucigenin can signal the presence of O-(2) even in systems not producing it in the absence of lucigenin. The reduction potential of lucigenin should be in accord with its ability to mediate O-(2) production; but it has not heretofore been measured in aqueous media. The problems facing such measurement are the insolubility of the divalently reduced form, which deposits on the electrode, and the slow conformational transition that follows the second electron transfer and which interferes with reversibility. We have now used rapid scan cyclic voltammetry to determine that the reduction potential for lucigenin is -0.14 +/- 0.02 V versus the normal hydrogen electrode. This value applies to both the first and the second electron transfers to lucigenin and it is in accord with the facile mediation of O-(2) production by this compound.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

January 15, 2000

Volume

373

Issue

2

Start / End Page

447 / 450

Location

United States

Related Subject Headings

  • Reactive Oxygen Species
  • Oxidation-Reduction
  • Molecular Structure
  • Luminescent Measurements
  • Electron Transport
  • Electrochemistry
  • Biochemistry & Molecular Biology
  • Acridines
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Spasojevic, I., Liochev, S. I., & Fridovich, I. (2000). Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production. Arch Biochem Biophys, 373(2), 447–450. https://doi.org/10.1006/abbi.1999.1579
Spasojevic, I., S. I. Liochev, and I. Fridovich. “Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production.Arch Biochem Biophys 373, no. 2 (January 15, 2000): 447–50. https://doi.org/10.1006/abbi.1999.1579.
Spasojevic I, Liochev SI, Fridovich I. Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production. Arch Biochem Biophys. 2000 Jan 15;373(2):447–50.
Spasojevic, I., et al. “Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production.Arch Biochem Biophys, vol. 373, no. 2, Jan. 2000, pp. 447–50. Pubmed, doi:10.1006/abbi.1999.1579.
Spasojevic I, Liochev SI, Fridovich I. Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production. Arch Biochem Biophys. 2000 Jan 15;373(2):447–450.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

January 15, 2000

Volume

373

Issue

2

Start / End Page

447 / 450

Location

United States

Related Subject Headings

  • Reactive Oxygen Species
  • Oxidation-Reduction
  • Molecular Structure
  • Luminescent Measurements
  • Electron Transport
  • Electrochemistry
  • Biochemistry & Molecular Biology
  • Acridines
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology