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Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse.

Publication ,  Journal Article
Urbas, A; Manning, MW; Daugherty, A; Cassis, LA; Lodder, RA
Published in: Anal Chem
July 15, 2003

Abdominal aortic aneurysms (AAAs) occur in 5-7% of people over age 60 in the United States. Early intervention in the disease process could have a significant impact on the incidence of complications and on patient survival, but identifying incipient aneurysms can be difficult. ApoE knockout mice develop AAAs following infusion of angiotensin II (AngII) by osmotic minipump into the subcutaneous space of mice at doses ranging from 500 to 1000 ng kg(-1) min(-1) for 7-28 days. These mice are used as models of AAA development. This study tested the hypothesis that near-IR spectrometry and PCR can determine AngII dose (SEE = 26 ng kg(-1) min(-1), SEP = 37 ng kg(-1) min(-1), r2 = 0.99) and collagen/elastin (C/E) ratio (SEE = 0.38, SEP = 0.39, r2 = 0.85) in mouse aortas.

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Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

July 15, 2003

Volume

75

Issue

14

Start / End Page

3650 / 3655

Location

United States

Related Subject Headings

  • Spectroscopy, Near-Infrared
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Elastin
  • Collagen
  • Calibration
  • Apolipoproteins E
  • Aortic Aneurysm, Abdominal
 

Citation

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Urbas, A., Manning, M. W., Daugherty, A., Cassis, L. A., & Lodder, R. A. (2003). Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse. Anal Chem, 75(14), 3650–3655. https://doi.org/10.1021/ac034113n
Urbas, Aaron, Michael W. Manning, Alan Daugherty, Lisa A. Cassis, and Robert A. Lodder. “Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse.Anal Chem 75, no. 14 (July 15, 2003): 3650–55. https://doi.org/10.1021/ac034113n.
Urbas A, Manning MW, Daugherty A, Cassis LA, Lodder RA. Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse. Anal Chem. 2003 Jul 15;75(14):3650–5.
Urbas, Aaron, et al. “Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse.Anal Chem, vol. 75, no. 14, July 2003, pp. 3650–55. Pubmed, doi:10.1021/ac034113n.
Urbas A, Manning MW, Daugherty A, Cassis LA, Lodder RA. Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse. Anal Chem. 2003 Jul 15;75(14):3650–3655.
Journal cover image

Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

July 15, 2003

Volume

75

Issue

14

Start / End Page

3650 / 3655

Location

United States

Related Subject Headings

  • Spectroscopy, Near-Infrared
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Elastin
  • Collagen
  • Calibration
  • Apolipoproteins E
  • Aortic Aneurysm, Abdominal