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Prediction of fertilization in vitro with human gametes: is there a litmus test?

Publication ,  Journal Article
Oehninger, S; Toner, J; Muasher, SJ; Coddington, C; Acosta, AA; Hodgen, GD
Published in: Am J Obstet Gynecol
December 1992

OBJECTIVES: The objectives of this study were as follows: (1) to evaluate the relationships between sperm concentration, morphologic pattern, motion parameters, and sperm-zona pellucida binding capacity and (2) to assess their ability to predict fertilization outcome under in vitro fertilization conditions. STUDY DESIGN: Semen samples from 44 infertile men were prospectively evaluated for density, morphologic pattern (strict criteria), computerized motion parameters (motility, velocity, and linearity), and hemizona assay (outcome expressed as hemizona assay index), and results were correlated with fertilization outcomes of preovulatory oocytes during in vitro fertilization. RESULTS: Of all sperm parameters, morphologic pattern was the best predictor of the ability of the sperm to bind to the zona pellucida. Hemizona assay index was the best predictor of fertilization rate. Stepwise regression analysis provided a model of hemizona assay index plus motility with highest predictability (R2 = 53.4%). CONCLUSIONS: (1) The influence of sperm morphologic pattern as a prognosticator of fertilization outcome is established in major part as a determinant of sperm binding ability to the zona pellucida; (2) the hemizona assay provides a robust index that is highly predictive of the potential of human gametes to achieve fertilization and highlights its use in infertility and contraception testing.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

December 1992

Volume

167

Issue

6

Start / End Page

1760 / 1767

Location

United States

Related Subject Headings

  • Treatment Outcome
  • Sperm-Ovum Interactions
  • Sperm Motility
  • Regression Analysis
  • Prospective Studies
  • Prognosis
  • Predictive Value of Tests
  • Obstetrics & Reproductive Medicine
  • Models, Biological
  • Male
 

Citation

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Oehninger, S., Toner, J., Muasher, S. J., Coddington, C., Acosta, A. A., & Hodgen, G. D. (1992). Prediction of fertilization in vitro with human gametes: is there a litmus test? Am J Obstet Gynecol, 167(6), 1760–1767. https://doi.org/10.1016/0002-9378(92)91772-3
Oehninger, S., J. Toner, S. J. Muasher, C. Coddington, A. A. Acosta, and G. D. Hodgen. “Prediction of fertilization in vitro with human gametes: is there a litmus test?Am J Obstet Gynecol 167, no. 6 (December 1992): 1760–67. https://doi.org/10.1016/0002-9378(92)91772-3.
Oehninger S, Toner J, Muasher SJ, Coddington C, Acosta AA, Hodgen GD. Prediction of fertilization in vitro with human gametes: is there a litmus test? Am J Obstet Gynecol. 1992 Dec;167(6):1760–7.
Oehninger, S., et al. “Prediction of fertilization in vitro with human gametes: is there a litmus test?Am J Obstet Gynecol, vol. 167, no. 6, Dec. 1992, pp. 1760–67. Pubmed, doi:10.1016/0002-9378(92)91772-3.
Oehninger S, Toner J, Muasher SJ, Coddington C, Acosta AA, Hodgen GD. Prediction of fertilization in vitro with human gametes: is there a litmus test? Am J Obstet Gynecol. 1992 Dec;167(6):1760–1767.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

December 1992

Volume

167

Issue

6

Start / End Page

1760 / 1767

Location

United States

Related Subject Headings

  • Treatment Outcome
  • Sperm-Ovum Interactions
  • Sperm Motility
  • Regression Analysis
  • Prospective Studies
  • Prognosis
  • Predictive Value of Tests
  • Obstetrics & Reproductive Medicine
  • Models, Biological
  • Male